| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Homer1 was used as the immunogen for the HOMER1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HOMER1 Antibody / SYN47 is a anti-HOMER1 Rabbit antibody Recombinant Rabbit Monoclonal clone 30H34 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: HOMER1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30H34, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, IP
Biological background
Homer protein homolog 1 binds to proline rich motifs in receptors and signaling proteins at the postsynaptic density. Research using HOMER1 antibody has demonstrated that it directly interacts with metabotropic glutamate receptors, inositol trisphosphate receptors, and Shank family proteins. These interactions assemble macromolecular signaling complexes that coordinate calcium dynamics and synaptic responses. Through these scaffolding functions, HOMER1 is essential for coupling surface receptors to intracellular calcium stores and signaling pathways.
Alternative splicing of the HOMER1 gene produces both constitutively expressed long forms and inducible short forms. Studies with HOMER1 antibody have revealed that the long isoforms provide structural stability, while the short isoform Homer1a is an immediate early gene product induced by neuronal activity. Homer1a functions as a dominant negative regulator that disrupts multimeric complexes, thereby promoting synaptic remodeling and plasticity. This dual regulation highlights the versatility of HOMER1 in controlling neuronal signaling.
Dysregulation of Homer protein homolog 1 has been associated with neurological and psychiatric disorders. Research using HOMER1 antibody has shown that altered expression contributes to conditions such as schizophrenia, autism spectrum disorders, and depression. Abnormal Homer1a induction has also been implicated in epilepsy models, where it disrupts synaptic signaling balance. These findings underscore the importance of HOMER1 in maintaining normal brain function.
In addition to neurological disorders, HOMER1 influences cardiovascular physiology. Studies with HOMER1 antibody have indicated that Homer protein homolog 1 participates in excitation contraction coupling in cardiomyocytes by regulating calcium signaling between the sarcoplasmic reticulum and plasma membrane. Dysregulation of HOMER1 in cardiac cells may contribute to arrhythmias and heart failure.
HOMER1 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting distinguishes isoform expression, immunohistochemistry demonstrates postsynaptic enrichment in brain tissue, and immunofluorescence highlights colocalization with synaptic markers. These applications make HOMER1 antibody valuable for research into synaptic physiology and pathology.
By supplying validated HOMER1 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
