| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Hsp70 was used as the immunogen for the Hsp70 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Hsp70 Antibody / Heat shock protein 70 / HSPA8 is a anti-HSP70 Rabbit antibody Recombinant Rabbit Monoclonal clone 31H87 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human.
Key elements and design rationale
- Target: HSP70
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31H87, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP, FACS
Biological background
Heat shock cognate protein 70 binds nascent polypeptides, unfolded proteins, and misfolded intermediates in an ATP dependent cycle. Research using Hsp70 antibody has shown that HSC70 cooperates with Hsp40 co chaperones and nucleotide exchange factors to stabilize client proteins, facilitate folding, and prevent aggregation. This activity is essential for maintaining proteome stability during protein synthesis and cellular stress.
Studies with Hsp70 antibody have revealed roles in intracellular protein transport. Heat shock cognate protein 70 participates in clathrin mediated endocytosis by uncoating clathrin coated vesicles, ensuring efficient trafficking of receptors and cargo. It also contributes to nuclear import, mitochondrial translocation, and protein transport across membranes. These findings highlight the broad role of HSC70 in protein logistics within the cell.
Beyond folding and trafficking, Heat shock cognate protein 70 is a central regulator of chaperone mediated autophagy. Research using Hsp70 antibody has demonstrated that HSC70 recognizes proteins containing KFERQ like motifs and directs them to lysosomes for degradation. This selective autophagy pathway maintains cellular homeostasis by removing damaged or superfluous proteins. Dysregulation of this pathway contributes to age related disease and neurodegeneration.
HSC70 also supports cellular survival under stress. Studies with Hsp70 antibody have shown that its levels increase during stress conditions, such as oxidative stress, heat shock, or metabolic imbalance. By stabilizing key signaling and structural proteins, HSC70 allows cells to adapt and recover from damage. Its protective function underscores why Hsp70 family proteins are considered central to stress tolerance mechanisms.
In disease contexts, dysregulation of Heat shock cognate protein 70 has been associated with cancer, infection, and neurodegeneration. Research using Hsp70 antibody has revealed that many tumor cells exploit HSC70 to maintain folding capacity under high metabolic stress, supporting uncontrolled growth. Pathogens, including viruses, hijack HSC70 to aid in replication and assembly. Conversely, in neurodegenerative disorders such as Alzheimer disease, Parkinson disease, and Huntington disease, insufficient chaperone activity allows toxic protein aggregates to accumulate. These findings make HSC70 both a biomarker and a potential therapeutic target.
Hsp70 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting quantifies expression and post translational modifications, immunohistochemistry reveals tissue distribution, and immunofluorescence demonstrates cytoplasmic and vesicular localization. These approaches make Hsp70 antibody indispensable for molecular chaperone research.
By providing validated Hsp70 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
