| Field | Specification |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human HSPA6 was used as the immunogen for the HSPA6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
HSPA6 Antibody / Heat shock 70 kDa protein 6 is a anti-HSPA6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human.
Key elements and design rationale
- Target: HSPA6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS
Biological background
Functionally, HSPA6 antibody identifies a 646-amino-acid cytoplasmic and nuclear chaperone that binds unfolded or misfolded polypeptides to prevent aggregation and promote refolding. HSPA6 operates through an ATP-dependent mechanism, alternating between substrate-binding and release cycles coordinated by its nucleotide-binding and substrate-binding domains. Unlike constitutive HSP70 family members, HSPA6 expression is strictly inducible and transient, making it a sensitive marker of acute cellular stress. It cooperates with co-chaperones such as HSP40 and nucleotide exchange factors to restore proteostasis during recovery from stress.
The HSPA6 gene is located on chromosome 1q23.3 and is transcriptionally regulated by heat shock factor 1 (HSF1). Expression is strongly induced by thermal stress and other cytotoxic insults in epithelial, neuronal, and endothelial cells. Under normal physiological conditions, HSPA6 levels are low or undetectable, but during stress, it rapidly accumulates in both the cytoplasm and nucleus, where it stabilizes denatured proteins and facilitates their refolding or degradation.
Pathologically, HSPA6 has been implicated in neuroprotection, cardiac stress response, and cancer cell survival. Upregulation in neurons protects against protein misfolding diseases such as Alzheimer's and Parkinson's, while elevated expression in tumors may contribute to resistance against apoptosis and chemotherapy. In cardiac tissues, HSPA6 supports survival under ischemic or oxidative stress conditions. Research using HSPA6 antibody supports studies in proteostasis, cellular stress pathways, and molecular chaperone biology.
HSPA6 antibody is validated for use in relevant research applications to detect inducible heat shock proteins and examine cellular responses to thermal or oxidative stress.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
