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HT-1080 cells, derived from the connective tissue of a 35-year-old male patient with Fibrosarcoma in 1972, are widely used for studying the mechanisms of tumor invasiveness and metastasis due to their highly aggressive and invasive nature.
HT-1080 cells have been extensively utilized in studies involving cell migration, invasion assays, and the testing of anti-cancer compounds. In the realm of therapeutic development, HT-1080 cells are employed in the screening of anti-cancer drugs and in the evaluation of their effects on cell viability, apoptosis, and metastatic potential.
HT-1080 cells have also been used in research focusing on the extracellular matrix, angiogenesis, and the role of various genes and proteins in cancer progression. HT-1080 cells produce matrix metalloproteinases (MMPs), enzymes that degrade components of the extracellular matrix and play a critical role in tumor invasion and metastasis. This feature makes the HT-1080 cell line useful for studies investigating the regulation of MMPs and their inhibitors.
In summary, the HT-1080 cell line, with its extensive applications in the study of cancer research, cell adhesion, migration, and invasion models, as well as in the development of therapeutic strategies, continues to be a valuable resource in cancer research.
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- Isoenzymes: G6PD, B
- Oncogenes: ras+
- Tumorigenic: Yes, in immunosuppressed mice
- Virus susceptibility: poliovirus 1, vesicular stomatitis (Indiana), RD114, feline leukemia virus (FeLV)
- Reverse transcriptase: negative
- Karyotype: Modal number: 2n=46, pseudodiploid
- cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
- supplements: Supplement the medium with 10% FBS and 1% NEAA
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 1 x 104 cells/cm2
- fluidRenewal: Every 3 days
- postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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