| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dipeptidyl peptidase 4;3.4.14.5 ;ADABP;Adenosine deaminase complexing protein 2;ADCP-2;Dipeptidyl peptidase IV;DPP IV;T-cell activation antigen CD26;TP103;CD26;Dipeptidyl peptidase 4 membrane form;Dipeptidyl peptidase IV membrane form;Dipeptidyl peptidase 4 soluble form;Dipeptidyl peptidase IV soluble form;DPP4;ADCP2, CD26; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: D34-P766 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma, Saliva, Urine |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human dipeptidyl peptidase 4 (gene DPP4) in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Dipeptidyl peptidase 4 (DPP4, also called CD26) is a cell surface serine protease that cleaves dipeptides from peptides with proline or alanine at the second position. It inactivates incretin hormones such as GLP-1 and GIP, and it acts as a costimulatory molecule on T cells. DPP4 is the target of gliptin drugs for type 2 diabetes and the receptor for MERS coronavirus.
Kit Components
| Anti-Human DPP4 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human DPP4 Standard | 2 vials, 20 ng/tube |
| Human DPP4 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine |
| Assay range | 312 pg/mL–20,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human DPP4 (Asp34–Pro766), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.5–6.4% (3 samples, n = 16 each) |
| Inter-assay CV | 4.9–8.3% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:100. In the supplier’s tests, serum and plasma samples measured 3127–734 ng/mL.
Example standard curve (OD450, ng/mL): 0 = 0.035; 0.312 = 0.101; 0.62 = 0.162; 1.25 = 0.3; 2.5 = 0.542; 5 = 0.928; 10 = 1.523; 20 = 2.176. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (ng/ml) | 0 | 0.312 | 0.62 | 1.25 | 2.5 | 5 | 10 | 20 |
| O.D. | 0.035 | 0.101 | 0.162 | 0.3 | 0.542 | 0.928 | 1.523 | 2.176 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 590 | 3074 | 9263 | 536 | 3241 | 9182 |
| Standard deviation | 26.55 | 196.73 | 574.3 | 26.26 | 269 | 661.1 |
| CV (%) | 4.5% | 6.4% | 6.2% | 4.9% | 8.3% | 7.2% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Njanja Enz et al. (2019) CD26/DPP4 - a potential biomarker and target for cancer therapy. Pharmacology & Therapeutics. 10.1016/j.pharmthera.2019.02.015
Gulati Seema et al. (2023) Beneficial effects of premeal almond load on glucose profile on oral glucose tolerance and continuous glucose monitoring: randomized crossover trials in Asian Indians with prediabetes. European Journal of Clinical Nutrition. 10.1038/s41430-023-01263-1
Wang Yueyue et al. (2022) Comprehensive analysis of key genes and pathways for biological and clinical implications in thyroid-associated ophthalmopathy. BMC Genomics. 10.1186/s12864-022-08854-5
Boccardi Virginia et al. (2015) Serum CD26 levels in patients with gastric cancer: a novel potential diagnostic marker. BMC Cancer. 10.1186/s12885-015-1757-0
Shiqing Shao et al. (2019) Diagnostic and prognostic significance of serum CD26 level in Asian women with high-grade serous ovarian carcinoma. Future Oncology. 10.2217/fon-2018-0725
Wang Xinhua et al. (2016) Prognostic Significance of Serum CD26 Concentration in Patients with Esophageal Squamous Cell Carcinoma. Archives of Medical Research. 10.1016/j.arcmed.2016.07.005
