| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Flt-3;Flk-2;STK-1;CD135;FLK2;FLT-3 |
| Conjugate | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Storage | |
| Target | |
| UniProt # |
Scientific Background
Flt-3(Receptor-type tyrosine-protein kinase FLT3) is also known as FLK-2(Fetal liver kinase-2), STK-1(Stem cell tyrosine kinase 1), CD135. FLT3 is a cytokine receptor which belongs to the receptor tyrosine kinase class III. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1.
Product Description
Mammalian (HEK293) Expression | Recombinant ProteinRecombinant FLT3 Human protein is produced in Mammalian (HEK293) expression system and supplied as lyophilized powder for stable long-term storage. Suitable for binding assays, SPR/BLI interaction studies, antibody development, ELISA development, and drug discovery applications.
Protein Specifications
| UniProt ID | P36888 |
|---|---|
| Expression System | Mammalian (HEK293) |
| Molecular Characterization | FLT3(Asn27-Ser543) hFc(Glu99-Ala330) 6×His tag |
| Amino Acids / Region | Asn27-Ser543 |
| Affinity Tag | C-Human Fc and 6×His Tag |
| Molecular Weight | 125-130 kDa |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyo… |
| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for… |
| Delivery | In Stock |
Safety & Handling
Research use only
This protein was produced in Mammalian (HEK293). Mammalian expression enables human-like glycosylation, correct disulfide bond formation, and proper protein folding — all critical for proteins used in binding interaction studies (SPR/BLI) and antibody characterisation. For transmembrane proteins expressed as ectodomains or nanodisc preparations, the expression system directly affects binding conformation and assay performance.
The molecular characterisation of this construct is: FLT3(Asn27-Ser543) hFc(Glu99-Ala330) 6×His tag. Cross-reference with the UniProt entry (P36888) to confirm the expressed region includes your domain of interest — receptor-binding domain, extracellular domain, or specific epitope — before ordering.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.. A gel image showing the protein band is provided with each lot on the Certificate of Analysis. This high-purity specification is suitable for SPR/BLI kinetics measurements, ELISA standard curve development, and antibody epitope binding studies.
Please refer to the Certificate of Analysis (CoA) for reconstitution instructions for your specific lot. Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (A Prepare single-use working aliquots after reconstitution to avoid repeated freeze-thaw cycles. Lyophilized protein is stable for 12 months at −80°C from the date of receipt.
Options listed in “availability content”; other statuses may take longer. Contact BioHippo to confirm current availability and place time-sensitive orders. For large-scale or bulk requirements, extended lead times may apply for custom production runs.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Alternative tag formats: Different Fc isotypes (hFc vs mFc), His-tag, or biotinylated variants may be available upon request.
- Biotinylated proteins: Site-specific biotinylation for SPR/BLI streptavidin-coated chip immobilisation.
- Nanodisc preparations: Full-length transmembrane protein in synthetic nanodisc format for soluble binding studies.
- Bulk quantities: mg-scale production for high-throughput screening.
- Custom species variants: Mouse, rat, or non-human primate orthologs may be available.
Contact BioHippo customer support for custom requirements.
