| Field | Specification |
|---|---|
| Target | |
| Alternative names | Chemokine (C-X3-C motif) ligand 1 ;cDNA, FLJ94424, Homo sapiens chemokine (C-X3-C motif) ligand 1 (CX3CL1), mRNA ;CX3CL1 ;hCG_15105 ; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: Q25-R339 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma, Saliva, Urine, Milk |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human chemokine (gene CX3CL1) in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Fractalkine (CX3CL1) is the only member of the CX3C chemokine family. It exists as a membrane-anchored adhesion molecule on endothelial cells and neurons and as a soluble chemoattractant after cleavage. Acting through CX3CR1, it guides monocyte, NK cell and T-cell adhesion and migration, and it mediates neuron-microglia communication in the brain.
Kit Components
| Anti-Human CX3CL1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human CX3CL1 Standard | 2 vials, 10 ng/tube |
| Human CX3CL1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <50 pg/mL |
| Standard | Recombinant human CX3CL1 (Gln25–Arg339), expressed in NS0 |
| Cross-reactivity | Below 0.2% cross-reactivity with Eotaxin, MCP-1, MCP-2, 6Ckine, MARC |
| Intra-assay CV | 5.7–6.4% (3 samples, n = 16 each) |
| Inter-assay CV | 6.0–6.6% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum samples measured 102–854 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.046; 156 = 0.112; 312 = 0.142; 625 = 0.24; 1250 = 0.43; 2500 = 0.76; 5000 = 1.27; 10000 = 1.871. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.046 | 0.112 | 0.142 | 0.24 | 0.43 | 0.76 | 1.27 | 1.871 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 296 | 1773 | 5345 | 269 | 1730 | 5847 |
| Standard deviation | 16.87 | 90.42 | 315.35 | 17.75 | 103.8 | 374.2 |
| CV (%) | 5.7% | 6.4% | 5.9% | 6.6% | 6% | 6.4% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Ketao Wang et al. (2021) Vertebral-specific activation of the CX3CL1/ICAM-1 signaling network mediates non-small-cell lung cancer spinal metastasis by engaging tumor cell-vertebral bone marrow endothelial cell interactions. Theranostics. 10.7150/thno.54235
You Mingfeng et al. (2022) Neuron derived fractalkine promotes microglia to absorb hematoma via CD163/HO-1 after intracerebral hemorrhage. Cellular and Molecular Life Sciences. 10.1007/s00018-022-04212-6
Jie Yang et al. (2026) Astragaloside IV directly targets PPP1R14B to sensitize prostate cancer to anti-PD-1 immunotherapy by remodeling the CX3CL1/CD8+ T cell axis. Phytomedicine. 10.1016/j.phymed.2026.158158
John D. Christie et al. (2021) Systemic delivery of TNF-armed myxoma virus plus immune checkpoint inhibitor eliminates lung metastatic mouse osteosarcoma. Molecular Therapy-Oncolytics. 10.1016/j.omto.2021.07.014
Tong Ming et al. (2020) Elevated Expression of Serum Endothelial Cell Adhesion Molecules in COVID-19 Patients. Journal of Infectious Diseases. 10.1093/infdis/jiaa349
Chi Sun et al. (2020) ADAM17-regulated CX3CL1 expression produced by bone marrow endothelial cells promotes spinal metastasis from hepatocellular carcinoma. International Journal of Oncology. 10.3892/ijo.2020.5045
Yan Lv et al. (2024) Differential alterations of CXCR3, CXCR5 and CX3CR1 in patients with immune thrombocytopenia. Cytokine. 10.1016/j.cyto.2024.156684
Ming Tong et al. (2022) Endothelial Biomarkers in Patients Recovered from COVID-19 One Year after Hospital Discharge: A Cross-Sectional Study. Mediterranean Journal of Hematology and Infectious Diseases. 10.4084/MJHID.2022.033
Xiangyu Wang et al. (2022) Vascular Protective Effects of Malus toringoides (Rehd.) Hughes Extracts and their Mechanism in Diabetic Rats and HUVECs. Evidence-based Complementary and Alternative Medicine. 10.1155/2022/4348435
Fei Fu et al. (2017) Identification of genes involved in enterovirus 71 infected SK-N-SH cells. International Journal of Clinical and Experimental Pathology. PMID: 31966515
