| Field | Specification |
|---|---|
| Target | |
| Alternative names | Insulin-like growth factor-binding protein 1;IBP-1;IGF-binding protein 1;IGFBP-1;Placental protein 12;PP12;IGFBP1;IBP1; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: A26-N259 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma, Urine |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human insulin-like growth factor-binding protein 1 (gene IGFBP1) in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
IGFBP-1 is a secreted insulin-like growth factor binding protein produced mainly by the liver and by decidualized endometrium. It binds IGF-1 and IGF-2 and controls their access to receptors, with levels acutely regulated by insulin and nutritional state, and it can also signal through integrin α5β1 to promote cell migration.
Kit Components
| Anti-Human IGFBP1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human IGFBP1 Standard | 2 vials, 10 ng/tube |
| Human IGFBP1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma (heparin, EDTA) and urine |
| Assay range | 31.2 pg/mL–2,000 pg/mL |
| Sensitivity | <1 pg/mL |
| Standard | Recombinant human IGFBP1 (Ala26–Asn259), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.9–7.1% (3 samples, n = 16 each) |
| Inter-assay CV | 5.4–8.8% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:20. In the supplier’s tests, serum and plasma samples measured 159–75205 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.03; 31.2 = 0.102; 62.5 = 0.161; 125 = 0.26; 250 = 0.445; 500 = 0.742; 1000 = 1.221; 2000 = 1.989. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.03 | 0.102 | 0.161 | 0.26 | 0.445 | 0.742 | 1.221 | 1.989 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 66 | 243 | 1016 | 68 | 225 | 1048 |
| Standard deviation | 3.23 | 17.25 | 67.05 | 3.67 | 19.8 | 86.98 |
| CV (%) | 4.9% | 7.1% | 6.6% | 5.4% | 8.8% | 8.3% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Cuicui Liu et al. (2018) Inflammation-Associated Cytokines IGFBP1 and RANTES Impair the Megakaryocytic Potential of HSCs in PT Patients after Allo-HSCT. Biology of Blood and Marrow Transplantation. 10.1016/j.bbmt.2018.01.027
Kang Nannan et al. (2022) Calpain7 negatively regulates human endometrial stromal cell decidualization in EMs by promoting FoxO1 nuclear exclusion via hydrolyzing AKT1. Biology of Reproduction. 10.1093/biolre/ioac041
Jiang Yue et al. (2016) Decreased expression of NR4A nuclear receptors in adenomyosis impairs endometrial decidualization. Molecular Human Reproduction. 10.1093/molehr/gaw042
Lijuan Zhou et al. (2024) IGFBP-rP1 is a potential therapeutic target in androgenic alopecia. Experimental Dermatology. 10.1111/exd.15024
Wanga Huajing et al. (2026) Human Embryo Exosomal miR-143-3p Impairs Decidualization by Targeting KRAS. Reproduction. 10.1093/reprod/xaag052
Xiaofang Xu et al. (2025) Inhibition of Hsp90 Alleviates Necroptosis and Inflammation in Lung Epithelial Cells During Pulmonary Ischemia–Reperfusion Injury. Clinical and Experimental Pharmacology and Physiology. 10.1111/1440-1681.70037
Xiaoyue Shen et al. (2013) Krüppel-like factor 12 negatively regulates human endometrial stromal cell decidualization. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2013.02.078
Li Juan et al. (2019) Activation of Uterine Smad3 Pathway Is Crucial for Embryo Implantation. Current Medical Science. 10.1007/s11596-019-2134-z
