| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-17A;IL-17;IL-17A;Cytotoxic T-lymphocyte-associated antigen 8;CTLA-8;IL17A;CTLA8, IL17; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: I20-A155 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human interleukin-17A (gene IL17A) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Interleukin-17A (IL-17A) is the founding member of the IL-17 cytokine family, produced mainly by Th17 cells, γδ T cells and innate lymphoid cells. Signaling through the IL17RA/IL17RC receptor and the adaptor ACT1 (TRAF3IP2), it induces chemokines, antimicrobial peptides and other inflammatory mediators, and it is a key target in psoriasis and autoimmune disease research.
Kit Components
| Anti-Human IL17A Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human IL17A Standard | 2 vials, 10 ng/tube |
| Human IL17A Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 31.2 pg/mL–2,000 pg/mL |
| Sensitivity | <1 pg/mL |
| Standard | Recombinant human IL17A (Ile20–Ala155), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.5–7.2% (3 samples, n = 16 each) |
| Inter-assay CV | 5.4–8.7% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured below the lowest standard.
Example standard curve (OD450, pg/mL): 0 = 0.056; 31.2 = 0.11; 62.5 = 0.156; 125 = 0.244; 250 = 0.457; 500 = 0.868; 1000 = 1.677; 2000 = 2.955. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.056 | 0.11 | 0.156 | 0.244 | 0.457 | 0.868 | 1.677 | 2.955 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 54 | 276 | 763 | 52 | 257 | 703 |
| Standard deviation | 3.88 | 19.59 | 34.33 | 4.52 | 18.5 | 37.96 |
| CV (%) | 7.2% | 7.1% | 4.5% | 8.7% | 7.2% | 5.4% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Isildar Basak et al. (2022) 2D and 3D cultured human umbilical cord-derived mesenchymal stem cell-conditioned medium has a dual effect in type 1 diabetes model in rats: immunomodulation and beta-cell regeneration. Inflammation and Regeneration. 10.1186/s41232-022-00241-7
Marco Romano et al. (2017) Mutations in JAK2 and Calreticulin genes are associated with specific alterations of the immune system in myelofibrosis. OncoImmunology. 10.1080/2162402X.2017.1345402
Charalampia Amerikanou et al. (2021) Linking the IL-17A immune response with NMR-based faecal metabolic profile in IBD patients treated with Mastiha. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2021.111535
Kong Xiufang et al. (2022) A comprehensive profile of chemokines in the peripheral blood and vascular tissue of patients with Takayasu arteritis. Arthritis Research & Therapy. 10.1186/s13075-022-02740-x
Wali Muhammad et al. (2024) NAC-Grafted ROS-Scavenging Polymer Nanoparticles for Modulation of Acute Lung Injury Microenvironment In Vivo. Biomacromolecules. 10.1021/acs.biomac.4c01290
Lunxu Liu et al. (2015) Interleukin-17 and Prostaglandin E2 Are Involved in Formation of an M2 Macrophage-Dominant Microenvironment in Lung Cancer. Journal of Thoracic Oncology. 10.1097/JTO.0b013e3182542752
Jiaojiao Chen et al. (2024) Geniposide alleviates imiquimod-induced psoriasis-like skin lesions in mice via inhibition of angiogenesis. International Immunopharmacology. 10.1016/j.intimp.2024.111923
Basak Isildar et al. (2024) Preconditioning of human umbilical cord mesenchymal stem cells with deferoxamine potentiates the capacity of the secretome released from the cells and promotes immunomodulation and beta cell regeneration in a rat model of type 1 diabetes. International Immunopharmacology. 10.1016/j.intimp.2024.111662
Sarker Rapty et al. (2023) Association of granulocyte macrophage colony-stimulating factor and interleukin-17 levels with obsessive–compulsive disorder: a case–control study findings. Scientific Reports. 10.1038/s41598-023-46401-8
Lijuan Mao et al. (2022) Associations between Autoimmunity and Depression: Serum IL-6 and IL-17 Have Directly Impact on the HAMD Scores in Patients with First-Episode Depressive Disorder. Journal of Immunology Research. 10.1155/2022/6724881
