| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | IL-6;MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor |
| Assay Time | |
| Assay Type | |
| Detection Range | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Sensitivity | |
| Target | |
| UniProt # |
Scientific background
IL6 (Interleukin 6) is an interleukin-family cytokine that helps coordinate immune cell communication and inflammatory responses.
Interleukin levels often shift quickly after immune stimulation, making them useful for time-course studies of inflammation and immune activation.
Quantifying interleukins can help interpret pathway engagement alongside phenotypic readouts (e.g., immune cell infiltration, fever, or tissue injury markers).
Why it matters
- Quantify IL6 (Interleukin 6) to compare biological changes across conditions, doses, or time points.
- Generate concentration data from a standard curve to support biomarker and mechanistic studies.
How the ELISA works
Designed for Human samples, this kit uses a The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL6 in the samples is then determined by comparing the OD of the samples to the standard curve.. After binding and washing, signal is converted to concentration using a standard curve.
Sample types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Detection range: 7.82-500 pg/mL
- Sensitivity/LoD: 3.3 pg/mL
- Assay time: 3.5h
Can?t Find What You?re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Small
Homocysteine-Responsive Covalent Organic Frameworks as Signaling Scaffolds: Modulating Transsulfuration for Depression Treatment
Oxidative Medicine and Cellular Longevity
Serum Lipopolysaccharide Is Associated with the Recurrence of Atrial Fibrillation after Radiofrequency Ablation by Increasing Systemic Inflammation and Atrial Fibrosis
Oxidative Medicine and Cellular Longevity
Vagus Nerve Stimulation Ameliorates Renal Ischemia-Reperfusion Injury through Inhibiting NF-κB Activation and iNOS Protein Expression
Biomedicine & Pharmacotherapy
JNK downregulation improves olanzapine-induced insulin resistance by suppressing IRS1Ser307 phosphorylation and reducing inflammation
Life Sciences
Icariin protects neurons from endoplasmic reticulum stress-induced apoptosis after OGD/R injury via suppressing IRE1α-XBP1 signaling pathway
Journal of Clinical Medicine
Elevated Circulating Endocan Levels Are Associated with Increased Levels of Endothelial and Inflammation Factors in Postprandial Lipemia
Journal of Ethnopharmacology
Integration of network pharmacology, bioinformatics and experimental verification strategy to discover the pharmacological mechanisms of mogroside acts against pharyngitis
Frontiers in Pharmacology
Xanthohumol ameliorates diabetic kidney disease through suppression of renal fibrosis by regulating SNHG10/miR-378b
Clinical Epigenetics
Clinical significance and prognostic value of SIRT1 genetic variants in sepsis: a multicenter hospital-based case–control study
Gut Pathogens
A triad of gut dysbiosis, dysregulated immunity, and ‘leaky’ gut characterize HCMV associated neonatal cholestasis
Journal of Inflammation Research
Alpha-Taxilin: A Potential Diagnosis and Therapeutics Target in Rheumatoid Arthritis Which Interacts with Key Glycolytic Enzymes Associated with Metabolic Shifts in Fibroblast-Like Synoviocytes
Microorganisms
Modulation of C. albicans-Induced Immune Response in Vaginal Epithelial Cells by Garcinoic Acid
Cancer Medicine
Anti-tumorigenic effects of naive and TLR4-primed adipose-derived mesenchymal stem cells on pancreatic ductal adenocarcinoma cells
Journal of Intensive Care
Low levels of CD72 and CD100 expression on circulating lymphocytes in immunosuppressive phase of sepsis is associated with mortality in septic patients
Genes
Exosomes derived from miRNA-210 overexpressing bone marrow mesenchymal stem cells protect lipopolysaccharide induced chondrocytes injury via the NF-κB pathway
Food Science & Nutrition
Penthorum chinense Pursh extract ameliorates hepatic steatosis by suppressing pyroptosis via the NLRP3/Caspase-1/GSDMD pathway
Heliyon
Silencing miR-155–5p expression improves intestinal damage through inhibiting inflammation and ferroptosis in necrotizing enterocolitis
Biological Trace Element Research
The Effect of Boric Acid and Calcium Fructoborate on T Helper Cell Differentiation by Influencing Foxp3 and Ror-γt in Rheumatoid Arthritis and Systemic Lupus Erythematosus
Biological Trace Element Research
The Effect of Boric Acid and Calcium Fructoborate on T Helper Cell Differentiation by Influencing Foxp3 and Ror-γt in Rheumatoid Arthritis and Systemic Lupus Erythematosus
BMC Complementary Medicine and Therapies
Piperine, a black pepper compound, induces autophagy and cellular senescence mediated by NF-κB and IL-6 in acute leukemia
Functional & Integrative Genomics
Unveiling the protective effects of BMSCs/anti-miR-124-3p exosomes on LPS-induced endometrial injury
Medical Oncology
Dual facets of MSC-derived small EVs: regulatory insights into antitumor mechanisms in pancreatic ductal adenocarcinoma
MOLECULAR BIOLOGY REPORTS
Cytokine profiles and metabolic dysregulation in endometriosis: insights into diagnostic and therapeutic targets
Clinica Chimica Acta
Predictive value of soluble fibrinogen-like protein 2 for survival in traumatic patients with sepsis
Reproductive Sciences
The Impact of Inflammatory Cytokines on Recurrent Pregnancy Loss: A Preliminary Investigation
bioRXiv
Radiation-induced non-neoplastic cells senescence promoting the proliferation and invasiveness of GBM through SASP/JAK2-STAT3 Pathway
Current Stem Cell Research & Therapy
Enhancing the Regenerative Potential of Adipose-Derived Mesenchymal Stem Cells Through TLR4-Mediated Signaling
CYTOTECHNOLOGY
Mechanism underlying the role of the circRNA OMA1/miR-654-3p/RAF1 axis in children with inflammatory bowel disease
Open Medicine
Significant role and the underly mechanism of cullin-1 in chronic obstructive pulmonary disease
Pediatric Health, Medicine and Therapeutics
Utilizing T-Lymphocyte Activation-Related Cytokines to Predict Non-Responsiveness to Treatment in Pediatric Kawasaki Disease
Genes & Genomics
Roles and action mechanisms of NRIP1 in pre-eclampsia
JOURNAL OF ASTHMA
STOML2 Knockdown Inhibits Inflammation and Airway Remodeling of PDGF-BB-Induced Airway Smooth-Muscle Cells by the MAPK Pathway
Cureus Journal of Medical Science
Relationship Between Serum Interleukin-6 Levels, Systemic Immune-Inflammation Index, and Other Biomarkers Across Different Rheumatoid Arthritis Severity Levels
Cureus Journal of Medical Science
Relationship Between Serum Interleukin-6 Levels, Systemic Immune-Inflammation Index, and Other Biomarkers Across Different Rheumatoid Arthritis Severity Levels
Journal of Environmental Biology
Impact of Pb and Cd exposure on oxidative stress and inflammatory markers in Baghdad fuel station workers: A case-control study
Neurological Sciences and Neurophysiology
Investigation of Pro-inflammatory and Anti-inflammatory Effects of Different Ω3/Ω6 PUFA Ratios on Alzheimer’s Model Induced with Amyloid β1-42 in Human SHSY5Y Cells by In vitro Methods
researchsquare
GhLIP1, a lipoic acid synthase gene, negatively regulates leaf senescence in cotton
Phytomedicine Plus
Inhibition of NOX4 by Cissus quadrangularis extract protects from Type 2 diabetes induced-steatohepatitis
bioRxiv
Exosomes derived from BMSCs with miR-124-3p inhibitor protects against LPS-induced endometritis through regulation of DUSP6, p-p65 and p-ERK
Journal of the Faculty of Medicine Baghdad
Changes in levels of Interleukin-6 (IL-6) and Interleukin-8 (IL-8) in the Serum of Preterm Delivery Pregnant Women Affected by Gingivitis
