| Field | Specification |
|---|---|
| Target | |
| Alternative names | Stromelysin-1;SL-1;3.4.24.17;Matrix metalloproteinase-3;MMP-3;Transin-1;MMP3;STMY1; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: Y18-C477 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human stromelysin-1 (gene MMP3) in cell culture supernatants, serum and plasma (heparin). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Stromelysin-1 (MMP-3) is a matrix metalloproteinase with broad specificity that degrades fibronectin, laminin, proteoglycans and several collagens. It also activates other MMPs such as pro-MMP-9, and it is widely studied in arthritis, tissue remodeling and neuroinflammation.
Kit Components
| Anti-Human MMP3 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human MMP3 Standard | 2 vials, 10 ng/tube |
| Human MMP3 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin) |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human MMP3 (Tyr18–Cys477), expressed in NS0 |
| Cross-reactivity | Detectable cross-reactivity with MMP-10 approximately 2%, and no detectable cross-reactivity with other MMPs |
| Intra-assay CV | 6.4–6.9% (3 samples, n = 16 each) |
| Inter-assay CV | 6.2–6.9% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Example standard curve (OD450, pg/mL): 0 = 0.01; 156 = 0.05; 312 = 0.082; 625 = 0.145; 1250 = 0.26; 2500 = 0.521; 5000 = 0.995; 10000 = 1.902. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.01 | 0.05 | 0.082 | 0.145 | 0.26 | 0.521 | 0.995 | 1.902 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 284 | 1539 | 4656 | 310 | 1656 | 4415 |
| Standard deviation | 14.48 | 100.03 | 297.98 | 19.22 | 112.6 | 304.63 |
| CV (%) | 6.9% | 6.5% | 6.4% | 6.2% | 6.8% | 6.9% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Amirkhizi Farshad et al. (2023) The association of vitamin D status with oxidative stress biomarkers and matrix metalloproteinases in patients with knee osteoarthritis. Frontiers in Nutrition. 10.3389/fnut.2023.1101516
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