| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neurotrophin-3;NT-3;HDNF;Nerve growth factor 2;NGF-2;Neurotrophic factor;NTF3; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: Y139-T257 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human neurotrophin-3 (gene NTF3) in cell culture supernatants and serum. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Neurotrophin-3 (NT-3, NTF3) is a secreted growth factor of the neurotrophin family that acts mainly through the TrkC receptor. It supports the survival and development of sensory neurons, including proprioceptive and visceral populations, and is studied in nervous system development, nerve injury and synaptic plasticity.
Kit Components
| Anti-Human NTF3 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human NTF3 Standard | 2 vials, 10 ng/tube |
| Human NTF3 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants and serum |
| Assay range | 15.6 pg/mL–1,000 pg/mL |
| Sensitivity | <2 pg/mL |
| Standard | Recombinant human NTF3 (Tyr139–Thr257), expressed in NS0 |
| Cross-reactivity | Below 2.5% cross-reactivity with NT-4; none detected with any other cytokine |
| Intra-assay CV | 4.1–6.9% (3 samples, n = 16 each) |
| Inter-assay CV | 5.4–8.0% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum samples measured below the lowest standard.
Example standard curve (OD450, pg/mL): 0 = 0.035; 15.6 = 0.074; 31.2 = 0.131; 62.5 = 0.211; 125 = 0.353; 250 = 0.66; 500 = 1.201; 1000 = 1.936. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.035 | 0.074 | 0.131 | 0.211 | 0.353 | 0.66 | 1.201 | 1.936 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 30 | 147 | 434 | 31 | 141 | 418 |
| Standard deviation | 1.83 | 6.02 | 29.94 | 2.32 | 7.61 | 33.44 |
| CV (%) | 6.1% | 4.1% | 6.9% | 7.5% | 5.4% | 8% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Janneke G.F. Hogervorst et al. (2021) Gestational acrylamide exposure and biomarkers of fetal growth: Probing the mechanism underlying the association between acrylamide and reduced fetal growth. Environment International. 10.1016/j.envint.2021.106668
Peter A. Forsyth et al. (2014) p75 Neurotrophin Receptor Cleavage by α- and γ-Secretases Is Required for Neurotrophin-mediated Proliferation of Brain Tumor-initiating Cells *. Journal of Biological Chemistry. 10.1074/jbc.M113.513762
Monalisa Jena et al. (2019) Effect of lurasidone vs olanzapine on neurotrophic biomarkers in unmedicated schizophrenia: A randomized controlled trial. Journal of Psychiatric Research. 10.1016/j.jpsychires.2019.02.007
Bilgiç Ayhan et al. (2016) Serum brain-derived neurotrophic factor, glial-derived neurotrophic factor, nerve growth factor, and neurotrophin-3 levels in children with attention-deficit/hyperactivity disorder. European Child & Adolescent Psychiatry. 10.1007/s00787-016-0898-2
Ge Li et al. (2018) Neurotrophin-3 released from implant of tissue-engineered fibroin scaffolds inhibits inflammation, enhances nerve fiber regeneration, and improves motor function in canine spinal cord injury. Journal of Biomedical Materials Research Part a. 10.1002/jbm.a.36414
Mishra Biswa Ranjan et al. (2019) Effect of Sertraline, Dosulepin, and Venlafaxine on Non-BDNF Neurotrophins in Patients With Depression: A Cohort Study. Journal of Clinical Psychopharmacology. 10.1097/JCP.0000000000001022
Yongyi Zhang et al. (2024) Platelet-rich plasma-derived exosomes enhance mesenchymal stem cell paracrine function and nerve regeneration potential. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2024.149496
Jiang Peng et al. (2010) Human umbilical cord Wharton's jelly-derived mesenchymal stem cells differentiate into a Schwann-cell phenotype and promote neurite outgrowth in vitro. Brain Research Bulletin. 10.1016/j.brainresbull.2010.12.013
Fatma Kalayci et al. (2017) The relationship of Chlamydophila pneumoniae with schizophrenia: The role of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) in this relationship. Revista Argentina de Microbiologia. 10.1016/j.ram.2016.09.009
Zhi-yuan Guo et al. (2015) Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms. Neural Regeneration Research. 10.4103/1673-5374.155442
