Human Pulmonary Mesenchymal Stem Cells (HPMSC)

Overview

Human Pulmonary Mesenchymal Stem Cells (HPMSC) is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Human Mesenchymal Stem Cells derived from Lung (Pulmonary) within the Respiratory system.

Mesenchymal stem cells (MSC) are a well-characterized population of adult stem cells. MSC have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. These properties, in combination with their developmental plasticity, have generated tremendous interest because of the potential use of MSC in regenerative medicine. Fetal lung has been identified as a rich source of MSC and studies have shown that MSC can differentiate into neural cells, in addition to their mesenchymal differentiation potential (Med)[1]. MSC have also been shown to enhance the engraftment of human umbilical cord blood-derived CD34 hematopoietic cells in immunodeficient mice [2]. Flow cytometric analysis showed that fetal lung MSC express CD13, CD29, CD44, CD90, CD105, CD166, and HLA-ABC. iXCells Biotechnologies provides high quality Human Pulmonary Mesenchymal Stem Cells (HPMSC), which are isolated from human lung tissue and cryopreserved at P1, with >0.5 million cells in each vial. HPMSC express CD73, CD90, and CD105. These HMPSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 12 population doublings in Mesenchymal Stem Cell Medium (Cat# MD-0037) under the condition suggested by iXCells Biotechnologies.

Key elements and design rationale

• Cell identity: Mesenchymal Stem Cells (Primary Cells, Custom Cells)
• Source context: Lung; Pulmonary; Respiratory
• Donor background: Age: Fetal, Adult
• Biosafety level: BSL-2 (follow your institution’s biosafety program and local regulations)

Product-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.

Biological background

Stem and progenitor cell models are widely used to study differentiation programs, lineage commitment, and regenerative responses under controlled culture perturbations.

Across primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.

Research relevance and current trends

• Increasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.
• Adoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.
• Integration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.

Common research applications

• Profile identity markers by flow cytometry or immunostaining in cultured cells
• Induce lineage differentiation and track marker changes over a maturation time-course
• Quantify functional responses to defined stimuli relevant to the model system
• Compare baseline phenotype across donors/conditions using gene expression profiling
• Evaluate multipotency using lineage-specific staining and gene expression panels

Interpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.

Notes for experimental interpretation

• Donor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.
• Passage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.
• Contamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.
• Use appropriate negative/positive controls for the readout (e.g., unstimulated controls, pathway agonists/antagonists) to contextualize observed changes.

SKU:BHC18500252

Customization & Add-ons: Can't find the cell line you need—or require a custom cell-based solution for your project? We can help you source the best match or support custom cell line services for diverse research needs, including cell line sourcing and selection (species, tissue, and disease model matching), stable cell line engineering (overexpression, knockdown, or knockout via CRISPR/Cas9, shRNA, or sgRNA), reporter gene integration (GFP, RFP, luciferase, and other fluorescent or bioluminescent constructs), genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles), inducible expression systems (Tet-On/Off and other regulatable constructs), drug resistance marker selection (puromycin, G418, hygromycin, and others), custom growth and media optimisation for specific assay requirements, scale-up production for high-throughput screening campaigns, and authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.