| Field | Specification |
|---|---|
| Target | |
| Gene ID | |
| UniProt # | |
| Species | |
| Applications | |
| Assay type | |
| Sample type(s) | Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant |
| Sensitivity | |
| Detection range | |
| Detection method | |
| Shelf life | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This kit quantifies human Secretin (SCT) by competitive ELISA. In this competitive-inhibition assay, analyte in the sample competes with a labeled conjugate for a limited number of antibody binding sites, so signal is inversely proportional to the concentration of Secretin (SCT) - the standard format for small analytes.
Performance Data
| Assay Type | Competitive ELISA |
|---|---|
| Detection Method | Colorimetric (450 nm, 540-570 nm reference) |
| Detection Range | 23.4-1500pg/mL |
| Sensitivity | 7.25pg/mL |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Species | Homo sapiens (Human) |
| Kit Versions | Traditional and Ready-to-use formats |
| Shelf Life | Traditional: 12 months | Ready-to-use: 16 months |
| Manufacturer No. | DL-SCT-Hu, DLR-SCT-Hu |
| Gene ID | 6343 |
| UniProt # | P09683 |
Kit Formats
This assay is offered in two formats: the Traditional kit (12-month shelf life) with standard reagent preparation, and the Ready-to-use kit (16-month shelf life) with pre-prepared components that reduce hands-on setup time. Both formats use the same validated antibody pair and deliver equivalent performance.
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Human ELISA kits from DLdevelop, or contact us for related assays and matched reagents for SCT research.
Can't Find What You're Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
