| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor necrosis factor ligand superfamily member 10;Apo-2 ligand;Apo-2L;TNF-related apoptosis-inducing ligand;Protein TRAIL;CD253;TNFSF10;APO2L, TRAIL; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: T95-G281 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma, Saliva |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human tumor necrosis factor ligand superfamily member 10 (gene TNFSF10) in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
TRAIL (TNFSF10, Apo2L) is a TNF family cytokine that induces apoptosis by engaging the death receptors DR4 (TNFRSF10A) and DR5 (TNFRSF10B). Its activity is tuned by decoy receptors, including TNFRSF10C, TNFRSF10D and osteoprotegerin, which bind TRAIL without triggering cell death. TRAIL is studied in cancer biology, immune surveillance and apoptosis research.
Kit Components
| Anti-Human TNFSF10 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human TNFSF10 Standard | 2 vials, 10 ng/tube |
| Human TNFSF10 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva |
| Assay range | 15.6 pg/mL–1,000 pg/mL |
| Sensitivity | <1 pg/mL |
| Standard | Recombinant human TNFSF10 (Thr95–Gly281), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.5–7.0% (3 samples, n = 16 each) |
| Inter-assay CV | 5.0–8.1% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured about 300 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.042; 15.6 = 0.13; 31.2 = 0.205; 62.5 = 0.343; 125 = 0.598; 250 = 1.024; 500 = 1.671; 1000 = 2.326. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.042 | 0.13 | 0.205 | 0.343 | 0.598 | 1.024 | 1.671 | 2.326 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 38 | 184 | 416 | 34 | 175 | 377 |
| Standard deviation | 2.66 | 9.56 | 18.72 | 2.75 | 9.62 | 18.85 |
| CV (%) | 7% | 5.2% | 4.5% | 8.1% | 5.5% | 5% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Li-Hua Peng et al. (2020) Engineering bacterial outer membrane vesicles as transdermal nanoplatforms for photo-TRAIL–programmed therapy against melanoma. Science Advances. 10.1126/sciadv.aba2735
Ting-Wei Gu et al. (2020) Outer membrane vesicles derived from E. coli as novel vehicles for transdermal and tumor targeting delivery. Nanoscale. 10.1039/D0NR03698F
Ruili Sun et al. (2011) Toll-like Receptor 3 (TLR3) Induces Apoptosis via Death Receptors and Mitochondria by Up-regulating the Transactivating p63 Isoform α (TAP63α) *. Journal of Biological Chemistry. 10.1074/jbc.M110.178798
Huong T X Nguyen et al. (2024) A Customizable Platform to Integrate CAR and Conditional Expression of Immunotherapeutics in T Cells. International Journal of Molecular Sciences. 10.3390/ijms251910568
Li Man et al. (2019) Exploiting tumor-intrinsic signals to induce mesenchymal stem cell-mediated suicide gene therapy to fight malignant glioma. Stem Cell Research & Therapy. 10.1186/s13287-019-1194-0
Qianxue Nie et al. (2017) Conjugation to 10 kDa Linear PEG Extends Serum Half-Life and Preserves the Receptor-Binding Ability of mmTRAIL with Minimal Stimulation of PEG-Specific Antibodies. Molecular Pharmaceutics. 10.1021/acs.molpharmaceut.6b00964
Li Man et al. (2019) Transforming Growth Factor-β Promotes Homing and Therapeutic Efficacy of Human Mesenchymal Stem Cells to Glioblastoma. Journal of Neuropathology and Experimental Neurology. 10.1093/jnen/nlz016
Yinong Duan et al. (2014) Schistosoma japonicum soluble egg antigens induce apoptosis and inhibit activation of hepatic stellate cells: a possible molecular mechanism. International Journal for Parasitology. 10.1016/j.ijpara.2013.11.003
