Human Umbilical Vein Endothelial Cells (HUVEC)

Overview

Human Umbilical Vein Endothelial Cells (HUVEC) is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Human Immune Cells AND Hematopoietic Cells derived from Umbilical cord (Umbilical Vein Endothelial) within the Cardiovascular system.

Human umbilical vein endothelial cells (HUVECs) are cells derived from the endothelium of veins from the umbilical cord. They are used as a laboratory model system for the study of the function and pathology of endothelial cells (e.g., angiogenesis) [1] . They are used due to their low cost, and simple techniques for isolating them from umbilical cords, which are normally resected after childbirth [2] . HUVECs can be easily made to proliferate in a laboratory setting. They exhibit a cobblestone phenotype when lining vessel walls. iXCells Biotechnologies provides high quality Human Umbilical Vein Endothelial Cells (HUVEC), which are isolated from human umbilical vein of mix donors, and cryopreserved at P2, with >0.5 million cells in each vial. HUVEC have “cobblestone” morphology and positive staining with vWF/Factor VIII and CD31. HUVEC are one of the mostly used cell types to study endothelial function in vitro, including angiogenesis [1] , signaling pathway under normal and pathological condition such as oxidative stress, hypoxia [2] and inflammation etc. These HUVEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi and can further expand no more than 3 passages in Endothelial Cell Growth Media under the condition suggested by iXCells Biotechnologies. Additional expansion may decrease the purity. Human umbilical vein endothelial cells (HUVECs) are cells derived from the endothelium of veins from the umbilical cord. They are used as a laboratory model system for the study of the function and pathology of endothelial cells (e.g., angiogenesis) [1] . They are used due to their low cost, and simple techniques for isolating them from umbilical cords, which are normally resected after childbirth [2] . HUVECs can be easily made to proliferate in a laboratory setting. They exhibit a cobblestone phenotype when lining vessel walls. iXCells Biotechnologies provides high quality Human Umbilical Vein Endothelial Cells (HUVEC), which are isolated from human umbilical vein of mix donors, and cryopreserved at P2, with >0.5 million cells in each vial. HUVEC have “cobblestone” morphology and positive staining with vWF/Factor VIII and CD31. HUVEC are one of the mostly used cell types to study endothelial function in vitro, including angiogenesis [1] , signaling pathway under normal and pathological condition such as oxidative stress, hypoxia [2] and inflammation etc. These HUVEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi and can further expand no more than 3 passages in Endothelial Cell Growth Media under the condition suggested by iXCells Biotechnologies. Additional expansion may decrease the purity.

Key elements and design rationale

• Cell identity: Immune Cells AND Hematopoietic Cells (Primary Cells)
• Source context: Umbilical cord; Umbilical Vein Endothelial; Cardiovascular
• Biosafety level: BSL-2 (follow your institution’s biosafety program and local regulations)

Product-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.

Biological background

Cells originating from the Cardiovascular system are commonly studied to understand tissue-specific physiology, signaling, and responses to perturbations in controlled in vitro settings.

Across primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.

Research relevance and current trends

• Increasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.
• Adoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.
• Integration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.
• Expansion of high-dimensional immune phenotyping and perturbation screens to map activation states and functional programs.

Common research applications

• Profile identity markers by flow cytometry or immunostaining in cultured cells
• Stimulate immune cells and quantify activation markers and cytokine release
• Quantify functional responses to defined stimuli relevant to the model system
• Compare baseline phenotype across donors/conditions using gene expression profiling
• Perform immune profiling by multiparameter flow cytometry to resolve major subsets

Interpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.

Notes for experimental interpretation

• Donor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.
• Passage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.
• Contamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.
• Use appropriate negative/positive controls for the readout (e.g., unstimulated controls, pathway agonists/antagonists) to contextualize observed changes.

SKU:BHC18500010

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