| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human ICAM1 / CD54 was used as the immunogen for the ICAM1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ICAM1 Antibody / CD54 is a anti-ICAM1 Rabbit antibody Recombinant Rabbit Monoclonal clone 32I16 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Mouse. Reported localization: Cell surface.
Key elements and design rationale
- Target: ICAM1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32I16, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
Intercellular adhesion molecule 1 plays a central role in leukocyte adhesion and transmigration across endothelial barriers. By binding LFA1 on lymphocytes and neutrophils, ICAM1 facilitates firm adhesion following rolling interactions mediated by selectins. Research using ICAM1 antibody has demonstrated that blockade of this interaction reduces immune cell recruitment, underscoring its role in inflammatory responses. This pathway is particularly important in autoimmune disease, infection, and transplant rejection, where excessive leukocyte infiltration contributes to pathology.
ICAM1 also contributes to signaling pathways that regulate cell survival, apoptosis, and cytoskeletal rearrangements. Engagement of ICAM1 initiates intracellular signaling cascades involving kinases and actin binding proteins. Studies with ICAM1 antibody have revealed that these signals promote endothelial barrier regulation, angiogenesis, and immune synapse formation. Because of these roles, Intercellular adhesion molecule 1 is more than a passive adhesion molecule; it actively shapes cellular communication and tissue dynamics.
In oncology, ICAM1 expression is altered in many cancers. Elevated ICAM1 on tumor cells can promote immune recognition, but in other contexts it facilitates tumor progression and metastasis by enhancing interactions with immune cells and endothelial barriers. Research with ICAM1 antibody has demonstrated that ICAM1 expression correlates with prognosis in melanoma, breast, and pancreatic cancer, making it both a biomarker and potential therapeutic target. Viral pathogens such as rhinoviruses also exploit ICAM1 as a receptor for entry, further highlighting its diverse biological importance.
ICAM1 antibody is applied in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. Western blotting confirms expression changes under inflammatory stimulation, while immunohistochemistry highlights localization in inflamed tissues and tumors. Flow cytometry with ICAM1 antibody allows quantification of surface expression on immune and endothelial cells. These applications provide critical insight into adhesion biology and immune regulation.
By supplying validated ICAM1 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
