| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Interleukin-10 was used as the immunogen for the IL10 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
IL10 Antibody / Interleukin 10 is a anti-IL10 Rabbit antibody Recombinant Rabbit Monoclonal clone AOIG-9 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: IL10
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone AOIG-9, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF, FACS
Biological background
Interleukin 10 functions through binding to the IL10 receptor complex, composed of IL10RA and IL10RB subunits, triggering activation of JAK1 and TYK2 kinases. This leads to phosphorylation of STAT3, which translocates to the nucleus to induce anti-inflammatory gene expression. Targets include SOCS3, IL1RA, and other regulatory molecules that dampen NF-?B-mediated transcription of inflammatory mediators such as TNF, IL1, and IL6. IL10 signaling is essential for balancing immune tolerance with pathogen defense.
The IL10 antibody is widely used in immunology, inflammation, and infectious disease research to study cytokine signaling, immune regulation, and autoimmune disorders. Western blot analysis detects a 19 kilodalton band corresponding to IL10, while ELISA and immunofluorescence applications reveal secretion patterns and localization in immune cell cultures. This antibody supports detailed characterization of IL10 expression in response to infection, inflammation, and therapeutic modulation.
Deficiency or dysregulation of IL10 signaling leads to chronic inflammation and autoimmune pathologies, including inflammatory bowel disease, rheumatoid arthritis, and psoriasis. Conversely, elevated IL10 levels in tumors and chronic infections can suppress immune responses and promote immune evasion. Therapeutic modulation of IL10 signaling is under investigation for treating autoimmune and inflammatory diseases, highlighting its dual protective and suppressive roles. The IL10 antibody provides a reliable tool for tracking cytokine expression, validating immunomodulatory pathways, and developing anti-inflammatory therapies.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
