| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human IL12B was used as the immunogen for the IL12B antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
IL12B Antibody / Interleukin 12 beta / IL12 p40 is a anti-IL12B Rabbit antibody Recombinant Rabbit Monoclonal clone 31I13 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human.
Key elements and design rationale
- Target: IL12B
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31I13, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF, FACS
Biological background
IL12B antibody is widely applied in immunology, inflammation, and translational medicine. By detecting IL12B, researchers can examine how immune signaling shapes host defense and pathology. Elevated IL12B expression contributes to autoimmune diseases such as psoriasis, Crohn disease, and multiple sclerosis. Conversely, defective IL12B leads to increased susceptibility to infection due to impaired Th1 responses.
ELISA quantifies IL12B in serum or culture supernatants. Western blot assays detect IL12B protein bands in activated immune cell lysates. Immunohistochemistry maps expression in lymphoid tissues, while immunofluorescence highlights secretion patterns in dendritic cells and macrophages. These diverse applications make IL12B antibody a versatile tool for immune research.
IL12B has therapeutic relevance as a target of monoclonal antibodies that block interleukin 12 and interleukin 23 signaling. Agents such as ustekinumab neutralize IL12B and provide clinical benefit in psoriasis and inflammatory bowel disease. By applying IL12B antibody, scientists can evaluate pathway activity and therapeutic responses in autoimmune and inflammatory disorders.
IL12B antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
