| Field | Specification |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human IL21R was used as the immunogen for the IL21R antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
IL21R Antibody / Interleukin-21 receptor is a anti-IL21R Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human. Reported localization: Cytoplasm, cell membrane.
Key elements and design rationale
- Target: IL21R
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS
Biological background
Functionally, IL21R antibody identifies a 538-amino-acid receptor composed of an extracellular cytokine-binding domain, a single transmembrane segment, and a cytoplasmic tail containing Box1 and Box2 motifs that recruit Janus kinases (JAK1 and JAK3). IL21R engagement by IL21 triggers STAT1 and STAT3 phosphorylation, promoting differentiation of B cells, T follicular helper cells, and cytotoxic CD8+ T cells.
The IL21R gene is located on chromosome 16p11.2 and is expressed primarily in lymphocytes, including B cells, natural killer cells, and T cells. Through IL21 signaling, IL21R regulates immunoglobulin production, cytotoxicity, and inflammatory cytokine release. It plays a key role in maintaining immune homeostasis and supporting antibody-mediated immunity.
Pathologically, mutations or altered expression of IL21R are associated with primary immunodeficiency, autoimmune diseases, and lymphoproliferative disorders. Loss of IL21R function impairs B and T cell responses, while overactivation contributes to chronic inflammation. Research using IL21R antibody supports studies in immunology, cytokine signaling, and immune regulation.
IL21R antibody is validated for use in western blotting, flow cytometry, and immunohistochemistry to detect receptor expression and signaling status.
Structurally, Interleukin-21 receptor shares homology with other gamma-chain cytokine receptors, featuring fibronectin type III-like domains for ligand binding. This antibody enables detailed analysis of IL21R's function in immune communication and adaptive responses.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
