| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human IL9 was used as the immunogen for the IL9 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
IL9 Antibody / Interleukin 9 is a anti-IL9 Rabbit antibody Recombinant Rabbit Monoclonal clone 31I10 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: IL9
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31I10, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Interleukin 9 was originally described as a T cell growth factor but has since been shown to have wide ranging effects. It promotes mast cell proliferation and activation, enhances antibody production by B cells, and stimulates mucus secretion by airway epithelial cells. Studies with IL9 antibody have demonstrated that this cytokine plays a key role in allergic diseases such as asthma, where IL9 driven mast cell and epithelial responses contribute to airway hyperreactivity. It also has roles in anti parasitic immunity, supporting host defense against helminths by promoting mast cell and eosinophil activity.
Dysregulation of IL9 production has been associated with chronic inflammatory diseases, autoimmune disorders, and cancer. Elevated IL9 levels are observed in asthma, atopic dermatitis, and inflammatory bowel disease, where it contributes to persistent inflammation and tissue remodeling. In oncology, IL9 can have both tumor promoting and anti tumor effects depending on context. Some studies using IL9 antibody have shown that it enhances tumor growth by supporting survival of malignant cells, while others suggest it can stimulate anti tumor immune responses. This dual role makes IL9 a target of interest for immunotherapy research.
IL9 antibody is commonly applied in ELISA, western blotting, immunohistochemistry, and flow cytometry. ELISA provides quantitative measurement of IL9 levels in serum and culture supernatants, while immunohistochemistry reveals tissue specific expression patterns. Flow cytometry with IL9 antibody enables detection of intracellular cytokine production in T helper subsets, offering insights into immune cell differentiation and function. These applications ensure that IL9 antibody remains versatile for both basic immunology and clinical research.
By supplying validated IL9 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
