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IMR-32 is a human neuroblastoma cell line derived from the adrenal medulla of a child diagnosed with neuroblastoma, a malignant tumor originating from neural crest cells. These cells exhibit characteristics of immature neuronal cells, making them a valuable model for studying neuronal differentiation, neuroblastoma pathogenesis, and the molecular mechanisms underlying neurodevelopmental processes. The IMR-32 cells have a high capacity for proliferation and retain the ability to synthesize catecholamines, particularly dopamine and norepinephrine, which are essential neurotransmitters in the nervous system.
IMR-32 cells display a diploid karyotype with specific chromosomal aberrations commonly associated with neuroblastoma, such as amplification of the MYCN oncogene. This feature makes them particularly useful for research into the genetic and molecular drivers of neuroblastoma, including MYCN's role in tumorigenesis and progression. Additionally, IMR-32 cells are employed in drug screening assays to evaluate the efficacy and cytotoxicity of potential therapeutic agents targeting neuroblastoma. However, it is crucial to note that these cells are intended solely for in vitro research purposes and are not suitable for any therapeutic or in vivo applications.
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- Isoenzymes: G6PD, B
- Virus susceptibility: Vesicular stomatitis (Indiana), herpes simplex, vaccinia, coxsackievirus B3, poliovirus 3 (poorly)
- Virus resistance: Echovirus 11
- Reverse transcriptase: Negative
- cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
- supplements: Supplement the medium with 10% FBS and 1% NEAA
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 1 x 104 cells/cm2
- fluidRenewal: Every 3 to 5 days
- postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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