| Field | Specification |
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| Host | |
| Immunogen | A synthetic peptide specific to Interleukin 1 beta protein was used as the immunogen for the Interleukin 1 beta antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Interleukin 1 beta Antibody / IL1B is a anti-IL1B Rabbit antibody Recombinant Rabbit Monoclonal clone 28I10 supplied in Purified format. Recommended for workflows such as Western blot (WB) with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: IL1B
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 28I10, isotype Rabbit IgG
- Format: Purified
- Applications (as listed): WB
Biological background
IL1B is a master regulator of immune responses. It promotes the release of secondary mediators including tumor necrosis factor alpha and interleukin 6, amplifying inflammatory cascades. By activating NF kappa B and MAP kinase pathways, interleukin 1 beta induces transcription of genes involved in cell survival, proliferation, and differentiation. In adaptive immunity, IL1B shapes T helper cell polarization and antibody production, linking innate signals to downstream immune functions.
Aberrant interleukin 1 beta signaling is associated with many diseases. Excessive production contributes to rheumatoid arthritis, inflammatory bowel disease, psoriasis, and gout. Elevated IL1B levels are also observed in cardiovascular disease, metabolic disorders such as type 2 diabetes, and neurodegenerative diseases where chronic inflammation worsens tissue injury. Because of its pathogenic potential, IL1B is a major therapeutic target, and several inhibitors have been developed to block its activity in clinical settings.
At the molecular level, IL1B exerts its effects by binding to interleukin 1 receptor type I, which recruits the receptor accessory protein IL1RAcP to form a signaling complex. This complex engages adaptors such as MyD88 and kinases like IRAK, culminating in NF kappa B and AP 1 activation. These transcription factors coordinate expression of inflammatory genes, ensuring rapid and sustained immune responses.
The Interleukin 1 beta antibody is used in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to detect both pro and mature forms of IL1B. These applications are essential for studying inflammasome activation, cytokine regulation, and the pathology of inflammatory disease. For researchers examining innate immunity, therapeutic blockade of cytokines, or chronic inflammation, the Interleukin 1 beta antibody is a reliable and specific reagent.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
