| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Integrin alpha-9/ITGA9 recombinant protein (Position: Q526-Q1035) was used as the immunogen for the ITGA9 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ITGA9 Antibody / Integrin alpha 9 is a anti-ITGA9 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: ITGA9
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Integrin alpha-9 is encoded by the ITGA9 gene located on chromosome 3p22.2. It is expressed in smooth muscle cells, fibroblasts, airway epithelium, and leukocytes. During embryonic development, Integrin alpha-9 contributes to lymphatic valve formation and respiratory system maturation. Loss of ITGA9 function in animal models leads to perinatal lethality due to chylothorax, underscoring its essential role in lymphatic and pulmonary development. In adult tissues, Integrin alpha-9 supports wound healing and inflammation by mediating adhesion of leukocytes to endothelial and epithelial cells.
The ITGA9 antibody is used in cell biology, cancer, and immunology research to study integrin-mediated adhesion and signaling. In immunohistochemistry, it detects membrane-associated staining in epithelial and stromal cells. Western blot analysis identifies a ~130 kilodalton band corresponding to the mature glycosylated protein. Integrin alpha-9 participates in epithelial-mesenchymal transition and tumor metastasis, where increased expression correlates with invasive behavior. Blocking ITGA9 function has been shown to suppress cancer cell migration and angiogenesis, highlighting its potential as a therapeutic target.
Integrin alpha-9 interacts with cytoplasmic partners such as talin and kindlin to transmit bidirectional signaling between the ECM and intracellular environment. Activation of this receptor regulates cellular responses including proliferation, differentiation, and immune recruitment. The ITGA9 antibody enables detailed analysis of these signaling events and provides insight into how integrin networks integrate mechanical and chemical cues during tissue remodeling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
