| Field | Specification |
|---|---|
| Mfr No | |
| Species |
The LC-540 cell line is an adherent cell model derived from an adult male Fischer rat. Known for its robust growth properties, this cell line has a modal chromosome number of 42, with a karyotypic range of 40 to 43. Approximately 21% of the cells exhibit aneuploidy, although no other structural abnormalities have been reported, indicating a relatively stable genomic profile.
LC-540 cells are tumorigenic, with the ability to form tumors when introduced into rats. This feature makes them particularly valuable for studying oncogenesis and tumor biology in a controlled in vitro environment. Additionally, these cells are susceptible to several viruses, including Herpes simplex virus, Vaccinia virus, Vesicular stomatitis virus, and Human poliovirus 1. This susceptibility renders LC-540 a useful model for virological research, particularly in exploring virus-host interactions, viral pathogenesis, and the development of antiviral strategies.
Due to their specific characteristics, LC-540 cells are instrumental in a range of research applications, including cancer research and virology, where they provide insights into the mechanisms of tumor formation and viral infections.
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- Tumorigenic: Yes, in rats
- Reverse transcriptase: positive
- Products: steroid hormone, estrogen (estradiol and others), androgen (testosterone and others)
- cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
- supplements: Supplement the medium with 10% FBS and 1% NEAA
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 1 to 2 x 106 cells/cm2
- fluidRenewal: 2 to 3 times per week
- postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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