| Field | Specification |
|---|---|
| Mfr No | |
| Species |
The LS147T cell line is a variant of LS-180, both of which are derived from a Duke's type B adenocarcinoma of the colon in a 58-year-old White female patient. The original LS-180 line was established by culturing the minced tumor tissue for 10 months. LS-147T, along with its parent line, is notable for its expression of multiple oncogenes including myc, myb, ras, and fos, while being negative for others like sis, abl, and ros. This line also expresses high levels of carcinoembryonic antigen (CEA), interleukin 6 (IL-6), and interleukin 10 (IL-10), which are important markers and potential targets in colorectal cancer research.
These cells exhibit several key characteristics of colonic epithelial cells, including abundant microvilli and intracytoplasmic mucin vacuoles, which are features typically associated with secretory cells in the colonic mucosa. Electron microscopy studies have confirmed these structural details, further supporting their origin and differentiation status. Importantly, LS-147T cells have been shown to be tumorigenic in immunodeprived mice, consistently producing tumors when inoculated subcutaneously at high cell densities, thus affirming their malignant potential.
Moreover, the LS-147T cell line is particularly valuable in studies focusing on the molecular and immunological aspects of colorectal cancer. It has been reported that this line is easier to subculture compared to its parent line, LS-180, making it a more practical choice for long-term studies. The robust production of CEA by these cells, which is significantly higher than that of other established lines like HT-29, makes LS-147T a critical model for understanding tumor marker dynamics and exploring targeted therapies in colorectal cancer.
—
- Protein expression: Colon Antigen 3 +, CEA +, p53 -, GFAP -, mRNA expression +
- Antigen expression: HLA A2, B13, B50, Blood type O
- Isoenzymes: ADA, 1: G6PD, B, PGM1, 1, PGM3, 2, PGD, A, ES-D, 1, PEP-D, 1
- Oncogenes: myc +, myb + , ras +, fos +, p53 +, sis -, abl -, ros -, src -
- Tumorigenic: Yes, in nude mice
- Reverse transcriptase: negative
- Products: Carcinoembryonic antigen (CEA) 1944 ng/106 cells in 10 days, mucin, interleukin-10 (IL-10), interleukin-6 (IL-6)
- Mutational profile: LS-174T cells carry a mutation in codon 12 of Kras gene: GGT(Wt Gly) >GAT(Asp)
- Karyotype: 45,x with one x chromosome missing but no other chromosomal aberrations
- cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
- supplements: Supplement the medium with 10% FBS and 1% NEAA
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 5 to 8 x 104 cells/cm2
- fluidRenewal: 2 to 3 times per week
- postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
10%
OFF
15%OFF
20%
OFF
$50
OFF
FREE
SAMPLE
