| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Amino acids KLQKKAKETPSVN were used as the immunogen for this Mcpt4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Mcpt4 antibody / Mast cell protease 4 is an antibody targeting MCPT4, raised in Goat for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: MCPT4.
- Antibody identity: Polyclonal (goat origin); Goat Ig.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Antigen affinity purified.
- Species reactivity: Mouse.
- Listed applications: IHC-P, ELISA (peptide) (refer to on-page specifications for application-specific guidance).
Biological background
Mcpt4 (Mast cell protease 4) is a protease enzyme that is predominantly found in mast cells, which are a type of white blood cell involved in the immune response. Proteases are enzymes that break down proteins into smaller peptides, and Mcpt4 is specifically known for its role in regulating inflammation and immune responses. Studies have shown that Mcpt4 can target a variety of proteins involved in the immune response, making it a key player in the body's defense against pathogens. One of the main functions of Mcpt4 is to regulate the release of pro-inflammatory mediators in the body. By targeting specific proteins involved in the immune response, Mcpt4 can help to modulate the body's inflammatory response, preventing excessive inflammation and tissue damage. In addition, Mcpt4 has also been shown to play a role in the activation of other immune cells, such as T cells and dendritic cells, further enhancing the body's ability to fight off pathogens and allergens. Due to its wide range of functions in the immune response, Mcpt4 has been implicated in a number of diseases: dysregulation of Mcpt4 levels has been linked to inflammatory disorders such as asthma, rheumatoid arthritis, and inflammatory bowel disease.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Immunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- ELISA: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
