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MDCK (Madin-Darby Canine Kidney) cells serve as a pivotal vitro model in pharmaceutical sciences, particularly in the study of epithelial transport, epithelial permeability, and as a tool for membrane permeability evaluation. These cells, originally derived from renal tubule cells of a canine, exhibit properties akin to enterocytes, making them an excellent absorption screening model and a reliable cell line for evaluating drug transport mechanisms.
MDCK cells are used to explore branching morphogenesis, a process crucial for understanding organ development and cellular differentiation. This capacity for complex organization underscores their relevance in studying epithelial tissue architecture and cellular accumulation.
MDCK cells are well-regarded for their ability to form tight, polarized epithelial layers, making them a valuable model for studying epithelial barrier function and cell polarity, making them an indispensable model for drug carrier systems and the study of intrinsic membrane permeability. The presence of apical membranes and well-defined cell junctions in MDCK cell monolayers facilitates detailed permeability experiments, enhancing our understanding of transepithelial secretion and the transport and metabolic functions inherent to epithelial cells.
In virology, MDCK cells are pivotal for studying human influenza viruses, such as the H3N2 strain, because they express receptors compatible with those viruses. This makes them a key resource for investigating the intricacies of viral infections, examining how epithelial cells react to viral challenges. Their utility extends to evaluating antiviral agents and vaccines, further emphasizing their significance in infectious disease research and therapeutic development.
In summary, MDCK cells are invaluable in pharmaceutical and virological research for their epithelial characteristics, transport studies, and utility in viral infection models, particularly for influenza viruses, making them indispensable in advancing our understanding of drug delivery, epithelial biology, and infectious diseases.
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- Virus susceptibility: Vesicular stomatitis (Indiana), vaccinia, coxsackievirus B5, reovirus 2, 3, adenovirus 4, 5, vesicular exanthema of swine, infectious canine hepatitis
- Virus resistance: Poliovirus 2, coxsackievirus B3, B4
- Reverse transcriptase: negative
- Products: Keratin
- cultureMedium: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 1 x 104 cells/cm2
- fluidRenewal: Every 3 days
- postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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