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The MH3924A cell line is a well-characterized model derived from Morris rat hepatoma 3924A, which is frequently used in research to study hepatocellular carcinoma (HCC). These cells have been extensively employed to investigate the mechanisms underlying HCC growth, metastasis, and therapeutic responses. In particular, MH3924A cells are noted for their robust proliferative capacity and their ability to invade surrounding tissues, making them a suitable in vitro and in vivo model for exploring cancer progression and potential treatments.
Studies have demonstrated that the proliferation and invasiveness of MH3924A cells can be significantly influenced by various factors. For instance, treatment with the immunosuppressive drug tacrolimus (FK506) has been shown to promote the proliferation of these cells, enhance their invasive potential, and increase the expression of key molecules involved in metastasis, such as CXCR4 and its ligand SDF-1α. FK506's effect on these cells underscores its potential to exacerbate cancer progression, particularly in the context of post-transplantation immunosuppression, where its use is common to prevent organ rejection but may inadvertently promote tumor growth.
Additionally, MH3924A cells have been genetically modified to express the human sodium/iodide symporter (hNIS), which significantly enhances their iodide uptake capability. This modification has facilitated the use of these cells in radioiodine therapy studies, providing insights into the potential application of gene therapy for targeting HCC. However, despite the increased uptake, the rapid efflux of iodide from the cells suggests that further modifications or combined treatments are necessary to retain the radioactivity within the tumor cells for effective therapy. The MH3924A cell line thus remains a pivotal model in both basic and applied cancer research, particularly in the study of HCC's molecular underpinnings and therapeutic strategies.
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- Tumorigenic: Yes, in ACI-rat
- Viruses: RAP-test negative by PCR for: Adenovirus FL, Adenovirus K87, Hantavirus, Kilham rat virus, Lmyfocytair choriomeningitis virus, Mycoplasma pulmonis, Pneumonia virus of mice, Rat corona virus / Sialoacryoadenitis virus, Rat parvo virus, Reovirus type 3, Sendai virus, Theiler-s encephalomyelitis virus, Toolan-s H-1 virus.
- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- doublingTime: 25 to 35 hours
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 2 x 104 cells/cm2
- fluidRenewal: Every 3 to 5 days
- postThawRecovery: Start culture using the complete contents of the cryovial in 2xT25 cell culture flasks. The cells will recover within 24 to 48 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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