| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived mouse Mmp12 recombinant protein (Position: K237-H442) was used as the immunogen for the Mmp12 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Mmp12 Antibody / Matrix metalloproteinase 12 is a anti-MMP12 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Mouse.
Key elements and design rationale
- Target: MMP12
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
MMP12 is predominantly expressed in macrophages, where it contributes to tissue turnover and inflammatory regulation by cleaving elastin, fibronectin, laminin, and type IV collagen. MMP12 antibody is often used in immunohistochemistry, western blot, and zymography to assess macrophage activation and ECM degradation activity. During chronic inflammation, such as in atherosclerosis, emphysema, and rheumatoid arthritis, overproduction of MMP12 accelerates ECM breakdown and tissue destruction. Conversely, controlled MMP12 activity is necessary for effective wound repair, pathogen clearance, and extracellular remodeling in normal immune responses.
Structurally, MMP12 contains the conserved catalytic zinc-binding motif (HEXXHXXGXXH) and a C-terminal hemopexin domain important for substrate specificity and interaction with tissue inhibitors of metalloproteinases (TIMPs). The MMP12 antibody is used to monitor expression and activity under various conditions where macrophage-mediated proteolysis contributes to disease pathology. The MMP12 gene is located on chromosome 11q22.2-q22.3, clustered with other MMP family members including MMP1, MMP3, and MMP13. The mature enzyme is secreted as a pro-MMP, which is activated by proteolytic cleavage or oxidative stress in inflamed tissues.
Beyond matrix degradation, MMP12 regulates cytokine bioavailability and macrophage migration, influencing the balance between inflammation and resolution. Elevated MMP12 levels have been correlated with chronic obstructive pulmonary disease (COPD) severity and vascular remodeling in atheromatous plaques. Genetic knockout models in mice have shown Mmp12 deletion reduces elastin breakdown and lung injury, highlighting its central role in tissue integrity. Research using MMP12 antibody supports studies of inflammation, fibrosis, tumor invasion, and innate immunity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
