| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Magnetic particles, carboxyl functionalized |
| Concentration | |
| Form | liquid |
| Product Type | |
| Relative Density | |
| Shipping | |
| Storage |
Overview
MonoMag Carboxyl Beads are magnetic beads with carboxylic acid groups. These surface groups allow covalent amide bond formation to antibodies, proteins, peptides or other biomolecules via primary amine (NH2) groups. Oligonucleotides, antibodies or other ligands with amine groups can be easily coupled to the beads. Activation through carbodimide is required. With their large surface area, uniform size, narrow size distribution and unique surface coating, MonoMag Carboxyl Beads exhibit high binding capacity and low non-specific binding of protein or nucleic acids. Their compact structure also ensures faster magnetic separation than Hi-Sur Mag Beads. We offer both hydrophilic and hydrophobicMonoMag Carboxyl Beads.
These’s MonoMag Carboxyl Beads are also available in an easy-to-use kit format to enable researchers to conjugate antibodies, proteins, peptides or other biomolecules of their own choice to these magnetic beads. With the engineered MonoMag Carboxyl Beads and the specifically formulated conjugation buffers, the resulting conjugates exhibit high binding capacity and low non-specific binding of protein or nucleic acids. The kit contains sufficient reagents & components for performing at least 5 conjugation reactions. All componets in the kit have been autoclaved. Briefly, the magnetic beads are activated using carbodiimide and N-hydroxysuccinimide followed by conjugation to amino groups that are present on the target protein/ligand. The recommended protocol has been used to successfully conjugate bovine serum albumin, streptavidin or immunoglobulin to these magnetic beads.
Key Features
- Significantly low non-specific binding to proteins and nucleic acid High binding capacity
- Immediate and gentle coupling at room temperature and neutral to low pH
- Narrow size distribution, CV≤5%
- No iron exposure to ensure lowest autosignal, particularly with respect to chemiluminescence
- Comparable performance with Dynabeads Myone Carboxylic Acid
Applications
- Immunoassay
- Immunoprecipitation
- Nucleic acid hybridization
- hybridization
- Western blot
- Cell separation
Physical & Chemical Properties
- Surface Functional Group: Carboxyl
- pH: 6.0 - 8.0 at 25 °C (77 °F)
- Water Solubility: Completely miscible
- Magnetic Content: ~35% (1 µm), ~25% (3 µm), ~18% (4.5 µm)
- Chemical Stability: Stable under recommended storage conditions.
- Appearance / Color: brown/black
MonoMag Carboxyl Beads are monodisperse superparamagnetic beads (1 µm, 3 µm, 4.5 µm; CV ≤ 5%) with carboxylic acid (–COOH) surface groups for EDC/NHS-mediated amine coupling. Both hydrophilic and hydrophobic 1 µm variants are offered to match different surface blocking and assay requirements.
Hydrophilic beads exhibit lower non-specific protein binding and are preferred for immunoassay and cell separation applications in aqueous biological buffers. Hydrophobic beads have a more hydrophobic surface that may facilitate passive protein adsorption in addition to covalent coupling — useful for applications where adsorption-based immobilization is acceptable.
EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide) activates the bead carboxyl groups to form an unstable o-acylisourea intermediate that reacts with primary amines. Adding NHS or Sulfo-NHS stabilizes the intermediate as an NHS ester, improving coupling efficiency at pH 7–8. The activation and coupling steps should be performed in amine-free buffer (e.g., MES pH 6.0 for activation, PBS pH 7.4 for coupling).
MonoMag beads (uniform 1–4.5 µm) are well-suited for automated liquid handling platforms that use magnetic separation plates or tube racks. Their consistent size ensures predictable separation times and reduces variability in automated workflows compared to polydisperse bead formats.
Yes — MonoMag Carboxyl Beads Conjugation Kits providing EDC, Sulfo-NHS, activation buffer, quenching buffer, and storage buffer are available separately. See the Documents section for the protocol PDF.
The following customization and add-on services are available for this product through the supplier. For inquiries and pricing, contact support@biohippo.com.
Customization Options
- Custom Particle Sizes: Magnetic beads in sizes beyond the standard catalog range, with narrow size distribution from nanometers to micrometers, can be manufactured for specific assay geometries and separation requirements.
- Custom Surface Functionalization: Customized surface coatings and functional group densities are available to optimize binding capacity, non-specific binding profiles, and colloidal stability for specific applications.
- Custom Conjugation Service: Pre-conjugated magnetic bead–antibody, bead–protein, or bead–streptavidin conjugates prepared using your supplied biomolecule are available. The supplier specializes in bioconjugation chemistry across magnetic beads, iron oxide nanoparticles, quantum dots, and latex beads.
- Assay Development Services: Support for magnetic bead-based chemiluminescent assay, lateral flow immunoassay, and custom immunoassay platform development is available.
- Bulk & OEM Manufacturing: OEM magnetic beads for chemiluminescent assay, lateral flow immunoassay, CTC (circulating tumor cell) isolation for liquid biopsy, and T-cell isolation and activation for immunotherapy are available at production scale.
To inquire about customization options, request a quote, or discuss OEM manufacturing, contact support@biohippo.com.
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