| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Chitotriosidase-1; Chitinase-1; Chit1 |
| Assay Time | |
| Assay Type | |
| Detection Range | |
| Expression System | |
| Gene ID | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: A22-S464 |
| Product Type | |
| Reactivity | |
| Sample Type(s) | cell culture supernatants, serum and plasma (heparin, EDTA). |
| Sensitivity | |
| Storage | |
| Target | |
| UniProt # |
Background
Also known as: Chitotriosidase-1, Chitinase-1, Chit1.
Mouse CHIT1 (Chit1) is a commonly measured biological analyte that can provide insight into cellular state and tissue physiology. This target is frequently investigated in Molecular & Cellular Biology research contexts. As with many protein targets, abundance can be influenced by transcriptional regulation, secretion or shedding, proteolytic processing, and clearance. Quantitative measurement is often used to connect molecular changes with phenotypes such as stress responses, immune activation, differentiation, or tissue remodeling.
Biological context and interpretation
Protein-level readouts complement nucleic-acid measurements by reflecting post-transcriptional control and protein stability. Depending on the model system, changes may be transient or sustained, and may represent direct pathway engagement or secondary effects. When interpreting results, consider sample matrix effects, timing relative to stimulation or treatment, and whether complexes or modified forms of the analyte may be present.
Why it matters in research
- Comparative quantification: Supports analysis across experimental groups, time points, or dose ranges.
- Pathway context: Useful as part of a broader marker panel to triangulate biological mechanisms.
- Model characterization: Helps profile baseline vs perturbed states in cells, tissues, or biofluids.
Related pathways and interacting partners
For many targets, interpretability improves when measured alongside biologically connected markers (e.g., upstream regulators, downstream effectors, and cell-type indicators). Designing panels around a pathway hypothesis can help distinguish primary pathway activation from general stress or inflammation.
Sample data
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.013 | 0.092 | 0.180 | 0.340 | 0.642 | 1.107 | 1.536 | 2.059 |
Kit components
| Catalog number | Description | Quantity | Storage of opened/reconstituted material |
|---|---|---|---|
| EZ2033-CA | Rat anti-mouse CHIT1 monoclonal antibody (Capture Antibody) | 500 µl, 4 µg/mL (recommended dilution 1:100) | Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. |
| EZ2033-DA | Biotinylated goat anti-mouse CHIT1 polyclonal antibody (Detection Antibody) | 500 µl (recommended dilution 1:100) | |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µl (recommended dilution 1:100) | |
| EZ2033-ST | Lyophilized recombinant mouse CHIT1 standard | 10 ng/tube×3 | Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. |
Materials required but not provided
- Microplate reader in standard size.
- Automated plate washer.
- Incubator.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- 96 well microplate (Cat# AR1100)
- Plate Sealers.
- Capture Antibody Diluent: PBS.
- Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- Stop Solution: 2 N H2SO4 (Cat# AR1105)
- Wash Buffer (PBS and PBS-T). Recipes:
- PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
- PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
- *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
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