| Field | Specification |
|---|---|
| Target | |
| Alternative names | Insulin-like growth factor I ;Igf1 ; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: G49-A118 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies mouse insulin-like growth factor 1 (gene Igf1) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Insulin-like growth factor 1 (IGF-1) is a peptide hormone, produced mainly by the liver under growth hormone control, that is structurally related to insulin. Acting through the IGF-1 receptor, it promotes cell growth, proliferation and survival and mediates many effects of growth hormone on body growth and metabolism.
Kit Components
| Anti-Mouse Igf1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Mouse Igf1 Standard | 2 vials, 10 ng/tube |
| Mouse Igf1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Mouse |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 62.5 pg/mL–4,000 pg/mL |
| Sensitivity | <5 pg/mL |
| Standard | Recombinant mouse Igf1 (Gly49–Ala118), expressed in E. coli |
| Cross-reactivity | No detectable cross-reactivity with IGF-2 |
| Intra-assay CV | 4.1–7.8% (3 samples, n = 16 each) |
| Inter-assay CV | 4.9–9.7% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:100. In the supplier’s tests, serum and plasma samples measured about 44–77 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.019; 62.5 = 0.069; 125 = 0.126; 250 = 0.239; 500 = 0.441; 1000 = 0.834; 2000 = 1.441; 4000 = 2.086. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.019 | 0.069 | 0.126 | 0.239 | 0.441 | 0.834 | 1.441 | 2.086 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 75 | 604 | 1350 | 69 | 617 | 1307 |
| Standard deviation | 5.85 | 24.76 | 93.15 | 6.69 | 30.23 | 92.79 |
| CV (%) | 7.8% | 4.1% | 6.9% | 9.7% | 4.9% | 7.1% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Shan Xiao et al. (2022) Serine metabolism orchestrates macrophage polarization by regulating the IGF1–p38 axis. Cellular & Molecular Immunology. 10.1038/s41423-022-00925-7
Yuanning Zheng et al. (2022) Early Dietary Exposures Epigenetically Program Mammary Cancer Susceptibility through Igf1-Mediated Expansion of the Mammary Stem Cell Compartment. Cells. 10.3390/cells11162558
Yue Liang et al. (2021) Generation and Characterization of a New Resistance to Thyroid Hormone Mouse Model with Thyroid Hormone Receptor Alpha Gene Mutation. Thyroid. 10.1089/thy.2019.0733
Saeed Y. Aghdam et al. (2021) Impairment of IGF-1 Signaling and Antioxidant Response Are Associated with Radiation Sensitivity and Mortality. International Journal of Molecular Sciences. 10.3390/ijms22010451
Haoqi Chen et al. (2026) Combined effects of a low-dose multi-target supplement (CaHMB, CBP, and HA) on delaying musculoskeletal aging. Food & Function. 10.1039/D6FO00352D
Schernthaner-Reiter Marie Helene et al. (2020) Prkar1a haploinsufficiency ameliorates the growth hormone excess phenotype in Aip-deficient mice. Human Molecular Genetics. 10.1093/hmg/ddaa178
Matz-Soja Madlen et al. (2014) Hepatic Hedgehog signaling contributes to the regulation of IGF1 and IGFBP1 serum levels. Cell Communication and Signaling. 10.1186/1478-811X-12-11
Xue-Qun Chen et al. (2007) Diversities in hepatic HIF-1, IGF-I/IGFBP-1, LDH/ICD, and their mRNA expressions induced by CoCl2 in Qinghai-Tibetan plateau mammals and sea level mice. American Journal of Physiology-Regulatory Integrative and Comparative Physiology. 10.1152/ajpregu.00397.2006
Jian-Hua Qu et al. (2011) Involvement of IGF-I signaling pathway in the regulation of steroidogenesis in mouse Leydig cells treated with fenvalerate. Toxicology. 10.1016/j.tox.2011.12.007
Zeng Qiangcheng et al. (2019) miR-29b-3p regulated osteoblast differentiation via regulating IGF-1 secretion of mechanically stimulated osteocytes. Cellular & Molecular Biology Letters. 10.1186/s11658-019-0136-2
