| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NDUFA1 recombinant protein (Position: M1-D70) was used as the immunogen for the NDUFA1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NDUFA1 Antibody / NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 1 is a anti-NDUFA1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria).
Key elements and design rationale
- Target: NDUFA1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
NDUFA1 is a small, highly conserved protein localized to the mitochondrial inner membrane. It associates with other accessory subunits of the alpha subcomplex, including NDUFA2 and NDUFA3, and is essential for the assembly and functional integrity of Complex I. By stabilizing the membrane domain, NDUFA1 ensures proper coupling of proton translocation with electron transfer, thereby contributing to the mitochondrial proton gradient and ATP synthesis. Mutations in NDUFA1 can impair Complex I activity, leading to mitochondrial encephalomyopathy, lactic acidosis, and neurodegenerative diseases.
The NDUFA1 antibody is widely used in mitochondrial biology, metabolism, and neurodegeneration research to study electron transport chain organization and oxidative phosphorylation. Western blot analysis identifies a 7 kilodalton band corresponding to NDUFA1, while immunofluorescence reveals mitochondrial localization colocalizing with markers such as TOM20 and COX IV. This antibody allows characterization of mitochondrial respiratory complexes under physiological and disease conditions.
Defects in Complex I assembly caused by NDUFA1 dysfunction contribute to reduced ATP production and excessive reactive oxygen species generation, triggering oxidative stress and apoptosis. The NDUFA1 antibody provides a key tool for investigating mitochondrial respiratory disorders, metabolic adaptation, and redox regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
