| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human NDUFB4 was used as the immunogen for the NDUFB4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NDUFB4 Antibody / NADH dehydrogenase ubiquinone 1 beta subcomplex subunit 4 is a anti-NDUFB4 Rabbit antibody Recombinant Rabbit Monoclonal clone 30N45 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: NDUFB4
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30N45, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
Although NDUFB4 does not directly participate in electron transfer, it contributes to the structural stability and assembly of Complex I. Research using NDUFB4 antibody has shown that accessory subunits help maintain the integrity of the complex, ensuring efficient electron flow and proton pumping. Loss or dysfunction of accessory proteins like NDUFB4 destabilizes Complex I, leading to reduced oxidative phosphorylation and ATP production. This highlights the importance of even non catalytic subunits in respiratory chain function.
Mutations and defects in Complex I subunits, including NDUFB4, are associated with mitochondrial diseases such as Leigh syndrome, encephalopathy, and mitochondrial myopathy. Research using NDUFB4 antibody has demonstrated that absence or reduction of NDUFB4 impairs assembly of Complex I, leading to decreased activity and energy deficiency. Such dysfunctions contribute to neuromuscular symptoms, developmental delays, and metabolic acidosis. Because mitochondrial disease mechanisms are complex, NDUFB4 antibody serves as an important tool for dissecting assembly defects and functional outcomes.
In cancer, altered expression of Complex I subunits including NDUFB4 has been observed. Tumor cells often exhibit metabolic reprogramming, favoring glycolysis over oxidative phosphorylation. However, subsets of tumors rely on intact mitochondrial respiration, making Complex I subunits potential biomarkers and therapeutic targets. Studies with NDUFB4 antibody have shown correlations between expression levels and tumor progression, underscoring its relevance in oncology. Beyond cancer, mitochondrial dysfunction involving NDUFB4 contributes to neurodegenerative diseases, metabolic disorders, and aging related decline.
NDUFB4 antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms protein abundance in mitochondria rich tissues such as brain, heart, and muscle. Immunohistochemistry highlights tissue distribution, while immunofluorescence demonstrates mitochondrial localization using colocalization with markers such as COX IV. These approaches ensure that NDUFB4 antibody is useful in both clinical and basic mitochondrial research.
By supplying validated NDUFB4 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
