| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | A synthesized peptide derived from human NDUFS2 was used as the immunogen for the NDUFS2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NDUFS2 Antibody / NADH dehydrogenase ubiquinone iron-sulfur protein 2 is a anti-NDUFS2 Rabbit antibody Recombinant Rabbit Monoclonal clone 30N44 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: NDUFS2
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30N44, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, IP
Biological background
NDUFS2 antibody is widely applied in mitochondrial biology, bioenergetics, and disease research. Complex I dysfunction is a hallmark of mitochondrial disorders, neurodegeneration, and metabolic disease. Detection of NDUFS2 provides a marker for complex I assembly and activity. By monitoring NDUFS2 expression, researchers can study mechanisms underlying mitochondrial dysfunction in conditions such as Leigh syndrome, Parkinson disease, and ischemia reperfusion injury.
Western blot assays detect NDUFS2 protein in mitochondrial fractions, while immunohistochemistry highlights expression in metabolically active tissues such as heart, brain, and muscle. Immunofluorescence reveals punctate mitochondrial staining consistent with its inner membrane localization. These applications allow comprehensive analysis of complex I structure and function.
Mutations in NDUFS2 cause severe mitochondrial disease characterized by impaired energy metabolism, developmental delay, and neurodegeneration. By applying NDUFS2 antibody, scientists can investigate the molecular basis of these disorders and evaluate therapeutic strategies aimed at restoring complex I activity.
Beyond rare genetic disease, NDUFS2 plays roles in hypoxic adaptation, aging, and cancer metabolism. Changes in NDUFS2 expression affect reactive oxygen species production and signaling, linking mitochondrial function to cellular stress responses. The antibody therefore provides a valuable reagent for broad applications in mitochondrial research.
NDUFS2 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
