| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NDUFS3 recombinant protein (Position: R26-K264) was used as the immunogen for the NDUFS3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NDUFS3 Antibody / NADH dehydrogenase ubiquinone iron-sulfur protein 3 is a anti-NDUFS3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria).
Key elements and design rationale
- Target: NDUFS3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Complex I, the largest enzyme complex of the electron transport chain, consists of more than 40 subunits organized into functional modules. NDUFS3 forms part of the peripheral arm that houses redox centers responsible for electron transfer. It interacts with other core subunits, including NDUFS2 and NDUFS7, during the assembly of catalytic intermediates. The NDUFS3 antibody supports detailed examination of these interactions, helping to elucidate how mitochondrial protein complexes coordinate ATP production and energy regulation.
Defects in NDUFS3 are associated with mitochondrial complex I deficiency, one of the most common causes of inherited metabolic disorders. Mutations can result in severe encephalomyopathy, cardiomyopathy, or Leigh syndrome, characterized by impaired oxidative phosphorylation and reduced ATP generation. The NDUFS3 antibody is a critical tool for studying such pathologies, allowing detection of expression defects and assessing the impact of genetic variants on mitochondrial function. Immunoblotting with this antibody reveals the presence and stability of Complex I subunits in patient-derived fibroblasts and model organisms.
In addition to its structural role, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 influences cellular redox balance and reactive oxygen species (ROS) generation. Dysregulation of Complex I components can contribute to oxidative stress, aging, and neurodegenerative diseases such as Parkinson's and Alzheimer's. The NDUFS3 antibody supports investigation of these mechanisms by enabling visualization of mitochondrial protein distribution and expression levels in tissues under oxidative stress or toxin exposure.
The NDUFS3 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry. It yields distinct mitochondrial staining, reflecting its subcellular localization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
