| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Amino acids M1-K274 from the human protein were used as the immunogen for the NQO1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
NQO1 Antibody is a anti-NQO1 Rabbit antibody Polyclonal (rabbit origin) supplied in Purified format. Recommended for workflows such as Western blot (WB), Immunofluorescence (IF), IHC-P, Flow cytometry (FACS) with listed reactivity in Human. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: NQO1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Purified
- Applications (as listed): WB, IF, IHC-P, FACS
Biological background
NQO1 expression is regulated by the Nrf2 pathway and is upregulated under stress conditions as part of the antioxidant response. Elevated levels of this enzyme have been reported in various cancers, suggesting a dual role in both cytoprotection and tumor progression. Studies using an NQO1 antibody have linked the protein to drug metabolism, chemoresistance, and its potential as a therapeutic target. Beyond oncology, NQO1 has been studied in relation to cardiovascular disease, neurodegeneration, and aging.
The enzyme also stabilizes certain proteins by protecting them from proteasomal degradation, further highlighting its regulatory importance in cell survival pathways. Given these diverse roles, the use of an NQO1 antibody is valuable for detecting protein expression across tissues, evaluating stress responses, and exploring disease-specific alterations.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
