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The P19 cell line, a type of pluripotent embryonal carcinoma, was initially obtained from a teratocarcinoma in a C3H/He strain mouse. This epithelial-like cell line exhibits the capability to clone at high proficiency when grown in a medium infused with 0.1mM β-mercaptoethanol. A notable feature of P19 cells is their adaptability to differentiate into neuronal and glial cells when exposed to retinoic acid. Simultaneously, they have the potential to transform into cardiac and skeletal muscle when exposed to dimethyl sulfoxide (DMSO). When subjected to both retinoic acid and DMSO, they predominantly show characteristics of retinoic acid-induced differentiation.
The P19 cell line has its origin in the mouse (Mus musculus) and belongs to the broad classification of Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, and Tetrapod. The cells embody the morphology of an epithelial tissue type derived from the embryo and are associated with the disease teratocarcinoma. They are primarily utilized in 3D cell culture applications within the product category of animal cells.
While cancer cells pose a significant health threat due to their rapid and aggressive growth, they also offer an invaluable resource for researchers studying cancer cell development and seeking more targeted treatments. In 1982, the P19 cell line was created when a 7.5-day mouse embryo was transplanted into a testis to induce tumour growth by McBurney and Rogers. They successfully isolated cell cultures from the primary tumour containing undifferentiated stem cells, termed embryonal carcinoma P19 cells. These cells demonstrated rapid growth without the need for feeder cells and were easy to maintain. Subsequent injection into blastocysts of another mouse strain confirmed the multipotency of P19 cells, as tissues from all three germ layers grew in the recipient mouse.
Several subtype cell lines have been derived from the original P19 cells, including P19S18, P19D3, P19RAC65, and P19C16. Each of these subtypes possesses unique differentiation capabilities into neuronal cells or muscle cells when treated with retinoic acid or DMSO, respectively. More recent studies have generated cell lines derived from differentiated P19 cells, which, owing to the pluripotency of P19 cells, can transform into ectoderm, mesoderm, and endoderm-like cells.
P19 cells are known for their sustained growth in serum-supplemented media. Their differentiation can be effectively controlled using nontoxic drugs such as retinoic acid, leading to the development of neurons, astroglia, and microglia. On the other hand, aggregates of P19 cells exposed to DMSO differentiate into endodermal and mesodermal derivatives, including cardiac and skeletal muscle. P19 cells are also amenable to transfection with DNA encoding recombinant genes, and stable lines expressing these genes can be conveniently isolated. This malleability and versatility make P19 cells an excellent resource for exploring the molecular mechanisms that govern the developmental decisions of differentiating pluripotent cells.
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Karyotype: n = 40, xY
- cultureMedium: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 degree Celsius for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. Do not allow the cells to remain confluent. Subculture at least every 48 hours.
- seedingDensity: Subculture at least every 48 hours
- fluidRenewal: Every 2 days
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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