| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | A synthesized peptide derived from human PDGF B was used as the immunogen for the PDGFB antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
| UniProt # |
Overview
PDGFB Antibody / Platelet derived growth factor subunit B is a anti-PDGFB Rabbit antibody Recombinant Rabbit Monoclonal clone 31P34 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PDGFB
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31P34, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Platelet derived growth factor subunit B is secreted as part of a larger precursor protein that undergoes proteolytic processing to yield the mature growth factor. Research using PDGFB antibody has demonstrated that PDGF-BB is a potent mitogen for fibroblasts, vascular smooth muscle cells, and pericytes. Its signaling regulates blood vessel development and maintenance, making it essential for embryonic angiogenesis and adult vascular remodeling. Knockout models lacking PDGFB develop severe vascular abnormalities, underscoring its importance in development.
Aberrant expression of Platelet derived growth factor subunit B has been linked to a wide range of diseases. Studies with PDGFB antibody have shown that overexpression promotes tumor growth and angiogenesis by stimulating stromal proliferation and vascular recruitment. In fibrotic disease, excess PDGFB drives fibroblast activation and extracellular matrix deposition. Conversely, deficiency in PDGFB signaling results in defective pericyte recruitment and fragile vasculature. These findings demonstrate its critical role in tissue integrity and pathology.
PDGFB also contributes to neurobiology. Research using PDGFB antibody has revealed that the protein supports neural progenitor proliferation and protects neurons from injury through trophic signaling. Dysregulation has been associated with neurodegenerative conditions and gliomas. Because of its diverse functions across tissues, Platelet derived growth factor subunit B remains an important therapeutic target.
PDGFB antibody is widely applied in western blotting, immunohistochemistry, and ELISA. Western blotting detects mature and precursor forms, immunohistochemistry highlights expression in vasculature and tumors, and ELISA quantifies secreted PDGFB in biological fluids. These applications make PDGFB antibody valuable in both basic and translational research.
By supplying validated PDGFB antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
