| Field | Specification |
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| Immunogen | A synthesized peptide derived from human Phospho-BCAR1 (Y410) was used as the immunogen for the Phospho-BCAR1 (Tyr410) antibody. |
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| Product Type | |
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| Reactivity | |
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Overview
Phospho-BCAR1 (Tyr410) Antibody / Breast cancer anti-estrogen resistance protein 1 is a anti-BCAR1 (phospho-Tyr410) Rabbit antibody Recombinant Rabbit Monoclonal clone 32B03 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: BCAR1 (phospho site: Tyr410)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32B03, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Phospho-BCAR1 (Tyr410) antibody is widely used to study integrin signaling, cytoskeletal dynamics, and cancer biology. BCAR1 phosphorylation at focal adhesions enables activation of Src family kinases and downstream effectors such as Rac and JNK. By detecting the phosphorylated form of BCAR1, this antibody provides insight into how cells sense and respond to extracellular matrix cues.
The antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, Phospho-BCAR1 (Tyr410) antibody detects phosphorylated isoforms distinct from non phosphorylated protein. Immunohistochemistry maps phosphorylation events in tissue samples, while immunofluorescence reveals spatial localization of activated BCAR1 at focal adhesions. These approaches allow researchers to monitor cell adhesion signaling with high precision.
BCAR1 phosphorylation has been linked to oncogenesis and therapy resistance. Elevated phospho BCAR1 levels are associated with enhanced tumor invasion and poor prognosis in breast and other cancers. By using Phospho-BCAR1 (Tyr410) antibody, scientists can evaluate how dysregulated adhesion signaling contributes to malignancy and whether Tyr410 phosphorylation serves as a biomarker of tumor progression.
Beyond oncology, BCAR1 phosphorylation plays roles in cardiovascular and immune systems. It regulates endothelial cell migration during angiogenesis and contributes to immune cell trafficking. In developmental contexts, BCAR1 activity is essential for morphogenesis and tissue patterning. The phospho specific antibody enables detailed investigation of these roles across models.
Phospho-BCAR1 (Tyr410) antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
