| Field | Specification |
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| Immunogen | A synthesized peptide derived from human Phospho-beta Catenin (T41 + S45) was used as the immunogen for the Phospho-Beta Catenin (Thr41/Ser45) antibody. |
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Overview
Phospho-Beta Catenin (Thr41/Ser45) Antibody is a anti-Beta Catenin (phospho-Thr41, Ser45) Rabbit antibody Recombinant Rabbit Monoclonal clone 32C69 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: Beta Catenin (phospho site: Thr41, Ser45)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32C69, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Phospho-Beta Catenin (Thr41/Ser45) antibody is widely used in cancer research, developmental biology, and signaling studies. Aberrant stabilization of beta catenin due to impaired phosphorylation leads to constitutive activation of Wnt signaling and is a hallmark of many cancers. By detecting phosphorylated beta catenin, this antibody enables monitoring of degradation signals and pathway regulation.
The antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence. Western blot assays with Phospho-Beta Catenin (Thr41/Ser45) antibody reveal phosphorylated bands distinct from non phosphorylated forms. Immunohistochemistry maps phospho beta catenin in tissues, while immunofluorescence highlights subcellular localization at membranes and cytoplasm under conditions of Wnt regulation. These applications provide high resolution insight into signaling events.
Phosphorylation of beta catenin at Thr41 and Ser45 is mediated by casein kinase I and glycogen synthase kinase 3, initiating a cascade of phosphorylation events that promote its degradation. Dysregulation of this process contributes to tumorigenesis, particularly in colorectal, liver, and ovarian cancers. By using Phospho-Beta Catenin (Thr41/Ser45) antibody, researchers can evaluate how phosphorylation dependent regulation of CTNNB1 impacts oncogenic signaling.
Beyond oncology, Wnt beta catenin signaling plays roles in stem cell biology, embryogenesis, and tissue regeneration. Monitoring phosphorylation at key sites provides mechanistic understanding of pathway control in diverse contexts. The phospho specific antibody therefore serves as a critical reagent for investigating developmental biology and regenerative medicine.
Phospho-Beta Catenin (Thr41/Ser45) antibody provided by
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
