| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-Chk1 (S280) was used as the immunogen for the Phospho-Chk1 (Ser280) antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Phospho-Chk1 (Ser280) Antibody / Checkpoint kinase 1 is a anti-CHK1 (phospho-Ser280) Rabbit antibody Recombinant Rabbit Monoclonal clone 31C46 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: CHK1 (phospho site: Ser280)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31C46, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Phospho-Chk1 (Ser280) antibody is widely used in DNA damage, cell cycle, and cancer research. By specifically detecting the phosphorylated form, it enables distinction between active checkpoint signaling and basal kinase levels. Chk1 activation at Ser280 supports ATR mediated responses to stalled replication forks, allowing cells to pause progression and repair damage.
The antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence. Western blot assays reveal bands corresponding to phosphorylated Chk1 distinct from unmodified forms. Immunohistochemistry maps phosphorylation patterns within tissues, while immunofluorescence highlights nuclear distribution during checkpoint activation. These tools allow precise monitoring of checkpoint signaling in experimental systems.
Dysregulation of Chk1 phosphorylation contributes to oncogenesis by enabling survival of cells with damaged DNA. Elevated phospho Chk1 levels have been observed in tumors, where they correlate with therapy resistance. By applying Phospho-Chk1 (Ser280) antibody, scientists can explore how checkpoint signaling influences cancer progression and therapeutic outcomes. It is also valuable for evaluating Chk1 inhibitors under development as cancer treatments.
Beyond cancer, Chk1 phosphorylation at Ser280 plays roles in embryonic development and stem cell biology, where it maintains genome stability during rapid proliferation. The phospho specific antibody provides a useful tool for probing these functions in developmental and regenerative contexts.
Phospho-Chk1 (Ser280) antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
