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| Immunogen | A synthesized peptide derived from human Phospho-eIF4EBP1 (T70) was used as the immunogen for the Phospho-eIF4EBP1 (Thr70) antibody. |
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Overview
Phospho-eIF4EBP1 (Thr70) Antibody / EIF4EBP1 is a anti-EIF4EBP1 (phospho-Thr70) Rabbit antibody Recombinant Rabbit Monoclonal clone 32E48 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: EIF4EBP1 (phospho site: Thr70)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32E48, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Eukaryotic translation initiation factor 4E binding protein 1 is one of several eIF4E binding proteins that control translation initiation in response to nutrient, growth factor, and stress signals. Research using Phospho-eIF4EBP1 (Thr70) antibody has demonstrated that Thr70 phosphorylation is a key regulatory step mediated by mTOR complex 1. This modification, along with phosphorylation at Thr37, Thr46, and Ser65, sequentially decreases binding to eIF4E. The cumulative effect is activation of translation initiation and enhanced protein synthesis, particularly for transcripts encoding growth-promoting and survival proteins.
Dysregulation of EIF4EBP1 phosphorylation has been linked to cancer, metabolic disease, and neurodegeneration. Studies with Phospho-eIF4EBP1 (Thr70) antibody have shown that hyperactivation of mTOR signaling leads to persistent phosphorylation of eIF4EBP1, contributing to uncontrolled translation and oncogenesis. In contrast, reduced phosphorylation in stress conditions suppresses translation, conserving resources. Because translation initiation is a central control point in gene expression, phospho-eIF4EBP1 serves as a valuable biomarker of cellular signaling status.
In oncology, high levels of phosphorylated eIF4EBP1 correlate with tumor progression, therapeutic resistance, and poor prognosis. Research using Phospho-eIF4EBP1 (Thr70) antibody has confirmed that cancer cells exploit mTOR-driven phosphorylation to promote protein synthesis needed for growth and survival. Inhibitors of mTOR restore eIF4EBP1 binding to eIF4E, suppressing translation and reducing tumor growth. These findings make detection of phosphorylation at Thr70 essential in both basic research and therapeutic monitoring.
Phospho-eIF4EBP1 (Thr70) antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting identifies phosphorylation state-specific bands, immunohistochemistry reveals phosphorylation in tumor tissues, and immunofluorescence demonstrates dynamic responses to nutrient and drug treatments. These methods make Phospho-eIF4EBP1 (Thr70) antibody a critical reagent for investigating translational control.
By supplying validated Phospho-eIF4EBP1 (Thr70) antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
