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| Immunogen | A synthesized peptide derived from human Phospho-ErbB 3 (Y1289) was used as the immunogen for the Phospho-ErbB3 (Tyr1289) antibody. |
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Overview
Phospho-ErbB3 (Tyr1289) Antibody / HER3 is a anti-ERBB3 (phospho-Tyr1289) Rabbit antibody Recombinant Rabbit Monoclonal clone 32E64 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: ERBB3 (phospho site: Tyr1289)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32E64, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Phospho-ErbB3 (Tyr1289) antibody is a critical reagent for studying growth factor receptor signaling. Aberrant activation of ErbB3 contributes to tumorigenesis and therapeutic resistance in cancers such as breast, ovarian, and gastric carcinoma. Elevated phosphorylation of ErbB3 is often associated with resistance to EGFR and HER2-targeted therapies, making detection of phospho-ErbB3 a valuable biomarker in oncology research.
The antibody is widely used in applications such as western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. In western blot assays, Phospho-ErbB3 (Tyr1289) antibody distinguishes phosphorylated receptor isoforms from total ErbB3 protein, allowing researchers to quantify receptor activation states. In immunohistochemistry, it provides tissue-level localization of phosphorylated ErbB3, highlighting differences between normal and cancerous samples. Immunofluorescence enables visualization of receptor activation in cultured cells, often in combination with markers of proliferation or survival pathways.
Phosphorylation at Tyr1289 plays a unique role in ErbB3 signaling because it directly recruits PI3K regulatory subunits, making this site essential for downstream signaling. Dysregulation of this phosphorylation leads to uncontrolled activation of the PI3K/AKT pathway, promoting survival and growth in tumor cells. Studies employing Phospho-ErbB3 (Tyr1289) antibody have shown that inhibition of ErbB3 phosphorylation can sensitize resistant tumors to kinase inhibitors, emphasizing its translational relevance.
Beyond oncology, ErbB3 signaling has been implicated in neural development and cardiac biology, where receptor tyrosine kinases regulate cell fate and tissue maintenance. The use of phospho-specific antibodies enables researchers to connect receptor activation with physiological and pathological outcomes in diverse tissues.
Phospho-ErbB3 (Tyr1289) antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
