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| Immunogen | A synthesized peptide derived from human Phospho-GLUT4 (S488) was used as the immunogen for the Phospho-GLUT4 (Ser488) antibody. |
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Overview
Phospho-GLUT4 (Ser488) Antibody / SLC2A4 is a anti-GLUT4 (phospho-Ser488) Rabbit antibody Recombinant Rabbit Monoclonal clone 32S34 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: GLUT4 (phospho site: Ser488)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32S34, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
GLUT4 is stored in intracellular vesicles under basal conditions and translocates to the plasma membrane in response to insulin stimulation. Research using Phospho-GLUT4 (Ser488) antibody has shown that phosphorylation at Ser488 modulates GLUT4 trafficking and activity, influencing how cells respond to hormonal signals. This modification connects insulin receptor activation with downstream control of glucose uptake.
Studies with Phospho-GLUT4 (Ser488) antibody have revealed that phosphorylation at Ser488 is mediated by kinases activated during insulin signaling, including Akt and related pathways. This modification fine tunes the balance between vesicular storage and surface localization, ensuring that glucose uptake matches metabolic demands. Dysregulation of GLUT4 phosphorylation impairs glucose handling and contributes to insulin resistance.
In type 2 diabetes and obesity, abnormal GLUT4 trafficking is a hallmark feature. Research using Phospho-GLUT4 (Ser488) antibody has demonstrated that altered phosphorylation disrupts GLUT4 translocation, leading to reduced glucose uptake in muscle and adipose tissue. This impairment contributes directly to hyperglycemia and metabolic imbalance. Targeting GLUT4 phosphorylation is being investigated as a strategy to restore glucose homeostasis in diabetes therapy.
Phospho-GLUT4 (Ser488) antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting distinguishes phosphorylated from total GLUT4 protein, immunohistochemistry localizes activated transporter in tissue sections, and immunofluorescence reveals dynamic trafficking patterns in cells. These applications make the antibody indispensable for metabolic research.
By providing validated Phospho-GLUT4 (Ser488) antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
