| Field | Specification |
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| Immunogen | A synthesized peptide derived from human Phospho-LRP6 (S1490) was used as the immunogen for the Phospho-LRP6 (Ser1490) antibody. |
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Overview
Phospho-LRP6 (Ser1490) Antibody / Low density lipoprotein receptor related protein 6 is a anti-LRP6 (phospho-Ser1490) Rabbit antibody Recombinant Rabbit Monoclonal clone 31L42 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: LRP6 (phospho site: Ser1490)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31L42, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Low density lipoprotein receptor related protein 6 is phosphorylated by kinases such as GSK3 and CK1 following Wnt ligand binding. Serine 1490 is one of the critical sites required for recruitment of the cytoplasmic effector Dishevelled and for inhibition of the beta catenin destruction complex. Research using Phospho-LRP6 (Ser1490) antibody has shown that phosphorylation at this site acts as a molecular switch, turning on downstream signaling. Without phosphorylation at serine 1490, Wnt mediated gene expression is severely impaired, demonstrating the importance of this modification in developmental and disease related signaling events.
Aberrant Wnt signaling is implicated in multiple cancers, including colorectal carcinoma, breast cancer, and hepatocellular carcinoma. Overexpression or hyperactivation of LRP6 contributes to increased beta catenin stabilization, driving oncogenic transcriptional programs. Studies employing Phospho-LRP6 (Ser1490) antibody have revealed that elevated phosphorylation correlates with poor prognosis and aggressive tumor progression. Beyond cancer, abnormal regulation of LRP6 phosphorylation is also associated with bone disorders, atherosclerosis, and neurodegenerative disease, highlighting the broad relevance of this receptor.
Phospho-LRP6 (Ser1490) antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting allows quantification of phosphorylated versus unphosphorylated forms, providing a readout of Wnt activation. Immunohistochemistry demonstrates tissue specific activation of Wnt signaling, while immunofluorescence visualizes membrane localized phosphorylation events in cell culture models. Researchers studying embryonic development, stem cell biology, and tumor progression use Phospho-LRP6 (Ser1490) antibody to dissect pathway activity with high specificity.
By providing validated reagents such as Phospho-LRP6 (Ser1490) antibody,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
