| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-PDGFR beta (Y1009) was used as the immunogen for the Phospho-PDGFR beta (Tyr1009) antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Phospho-PDGFR beta (Tyr1009) Antibody is a anti-PDGFR (phospho-Tyr1009) Rabbit antibody Recombinant Rabbit Monoclonal clone 32P51 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: PDGFR (phospho site: Tyr1009)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32P51, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Phospho-PDGFR beta (Tyr1009) antibody is widely applied in cancer biology, cardiovascular research, and developmental biology. PDGFR beta is essential for vascular development, pericyte recruitment, and wound healing. Aberrant phosphorylation contributes to oncogenesis, fibrotic disease, and abnormal angiogenesis. By detecting phosphorylation at Tyr1009, researchers can assess PDGFR beta activation status in normal and pathological contexts.
Western blot assays detect phosphorylated PDGFR beta distinct from unmodified protein. Immunohistochemistry maps receptor activation in tumors and vascular tissues, while immunofluorescence highlights membrane localization at sites of ligand stimulation. These methods allow precise analysis of receptor phosphorylation in situ.
Aberrant PDGFR beta phosphorylation has been linked to glioblastoma, leukemia, and sarcomas. Targeted therapies such as tyrosine kinase inhibitors aim to suppress PDGFR signaling, and phospho specific antibodies provide biomarkers for drug efficacy. By applying Phospho-PDGFR beta (Tyr1009) antibody, scientists can investigate therapeutic mechanisms, evaluate resistance pathways, and develop improved interventions.
PDGFR beta phosphorylation is also important in cardiovascular disease and tissue repair. It regulates smooth muscle proliferation, vascular remodeling, and fibrotic responses. Dysregulation leads to hypertension, atherosclerosis, and organ fibrosis. The phospho-specific antibody therefore supports research across oncology, cardiology, and regenerative medicine.
Phospho-PDGFR beta (Tyr1009) antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
