| Field | Specification |
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| Immunogen | A synthesized peptide derived from human Phospho-Rb (S780) was used as the immunogen for the Phospho-Rb (Ser780) antibody. |
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| Reactivity | |
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Overview
Phospho-Rb (Ser780) Antibody / Retinoblastoma is a anti-Rb (phospho-Ser780) Rabbit antibody Recombinant Rabbit Monoclonal clone 31R44 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: Rb (phospho site: Ser780)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31R44, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Retinoblastoma associated protein functions as a gatekeeper of the G1 to S phase transition. Research using Phospho-Rb (Ser780) antibody has shown that phosphorylation at serine 780, mediated by cyclin dependent kinases such as CDK4 and CDK6, inactivates its ability to bind E2F transcription factors. This releases E2F, allowing transcription of genes required for DNA synthesis. Thus, phosphorylation at Ser780 is a critical step in promoting cell cycle progression.
Studies with Phospho-Rb (Ser780) antibody have revealed that this modification integrates growth factor signals with cell cycle machinery. In proliferating cells, growth signals activate cyclin D/CDK4 complexes, which phosphorylate Rb at serine 780. In contrast, in quiescent cells or under growth arrest conditions, Rb remains hypophosphorylated and bound to E2F, maintaining transcriptional repression. This phosphorylation-dependent switch ensures precise regulation of proliferation.
Dysregulation of Retinoblastoma associated protein phosphorylation contributes to cancer. Research using Phospho-Rb (Ser780) antibody has demonstrated that hyperphosphorylation leads to loss of tumor suppressor function, uncontrolled cell cycle entry, and oncogenic transformation. Mutations or deletions of RB1 similarly disrupt its ability to restrain E2F activity, resulting in tumorigenesis. Monitoring phosphorylation at Ser780 provides critical information about cell cycle deregulation in cancer.
Phospho-Rb (Ser780) antibody is widely used in western blotting, immunohistochemistry, and flow cytometry. Western blotting distinguishes phosphorylated from unmodified Rb, immunohistochemistry reveals activated cell cycle regions in tissues, and flow cytometry quantifies phosphorylation dynamics in cell populations. These applications make the antibody indispensable for cancer biology and cell cycle research.
By supplying validated Phospho-Rb (Ser780) antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
