| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide specific to human Tau / MAPT (surrounding pS324) was used as the immunogen for the Phospho-Tau antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Phospho-Tau Antibody pS324 is a anti-Tau Rabbit antibody Recombinant Rabbit Monoclonal clone 17M40 supplied in Purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Human.
Key elements and design rationale
- Target: Tau
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 17M40, isotype Rabbit IgG
- Format: Purified
- Applications (as listed): WB, IHC-P
Biological background
Phosphorylation at serine 324 occurs within the proline rich domain of tau, a region critical for microtubule binding and protein interactions. Modification at this site has been associated with reduced microtubule stabilization and altered neuronal morphology. By using phospho specific antibodies, researchers can track dynamic phosphorylation changes that affect tau function, misfolding, and aggregation. These measurements are essential for understanding how phosphorylation contributes to tau driven pathology.
In disease contexts, hyperphosphorylation of tau leads to its detachment from microtubules, promoting aggregation into paired helical filaments and neurofibrillary tangles. These pathological structures disrupt axonal transport and synaptic communication, contributing to cognitive decline in Alzheimer disease and related dementias. Phosphorylation at serine 324 is one of many modifications linked to this process, highlighting the need for site specific detection tools.
At the molecular level, tau phosphorylation is regulated by kinases such as GSK3 beta, CDK5, and MAP kinases, as well as phosphatases that reverse the modification. The balance of these enzymes determines tau stability and aggregation propensity. Antibodies against phosphorylated residues such as pS324 enable scientists to monitor this regulatory balance and assess the impact of kinase activity in cellular and animal models.
The Phospho Tau antibody pS324 is widely used in western blotting, immunohistochemistry, immunofluorescence, and ELISA to detect site specific phosphorylation. These applications provide insights into disease mechanisms, biomarker discovery, and therapeutic testing. For researchers studying tauopathies, neuronal cytoskeleton regulation, or phosphorylation signaling, this antibody provides a sensitive and specific detection tool.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
